Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/blaCMY-2 resistance plasmids

E. S. Berg, A. L. Wester, Johanne Ahrenfeldt, S. S. Mo, J. S. Slettemeås, M Steinbakk, Ø. Samuelsen, N. Grude, G. S. Simonsen, I. H. Løhr, S. B. Jørgensen, S. Tofteland, Ole Lund, U. R. Dahle, M. Sunde

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    Abstract

    In 2012 and 2014 the Norwegian monitoring programme for antimicrobial resistance in the veterinary and food production sectors (NORM-VET) showed that 124 of a total of 406 samples (31%) of Norwegian retail chicken meat was contaminated with extended-spectrum cephalosporin-resistant Escherichia coli. The aim of this study was to compare selected cephalosporin-resistant E. coli from humans and poultry to determine their genetic relatedness based on whole genome sequencing (WGS). E. coli representing three prevalent cephalosporin-resistant multi-locus sequence types (STs) isolated from poultry (n=17) were selected from the NORM-VET strain collections. All strains carried an IncK plasmid with a blaCMY-2 gene. Clinical E. coli isolates (n=284) with AmpC-mediated resistance were collected at Norwegian microbiology laboratories from 2010 to 2014. PCR screening showed that 29 of the clinical isolates harboured both IncK and blaCMY-2. All IncK/blaCMY-2 positive isolates were analysed by WGS-based bioinformatics tools. Analysis of single nucleotide polymorphisms (SNP) in 2.5 Mbp of shared genome sequences showed close relationship with less than 15 SNP differences between five clinical isolates from urinary tract infections, and the ST38 isolates from poultry. Furthermore, all of the 29 clinical isolates harboured IncK/blaCMY-2 plasmid variants highly similar to the IncK/blaCMY-2 plasmid present in the poultry isolates. Our results provide support for the hypothesis that clonal transfer of cephalosporin-resistant E. coli from chicken meat to humans may occur, and may cause difficult to treat infections. Furthermore, these E. coli can be a source of AmpC resistance plasmids for opportunistic pathogens in the human microbiota.
    Original languageEnglish
    JournalClinical Microbiology and Infection
    Volume23
    Issue number6
    ISSN1198-743X
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Zoonotic transfer of cephalosporin resistant E. coli
    • Chicken meat
    • Plasmid-mediated AmpC transmission
    • Urinary tract infections

    Cite this

    Berg, E. S. ; Wester, A. L. ; Ahrenfeldt, Johanne ; Mo, S. S. ; Slettemeås, J. S. ; Steinbakk, M ; Samuelsen, Ø. ; Grude, N. ; Simonsen, G. S. ; Løhr, I. H. ; Jørgensen, S. B. ; Tofteland, S. ; Lund, Ole ; Dahle, U. R. ; Sunde, M. / Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/blaCMY-2 resistance plasmids. In: Clinical Microbiology and Infection. 2017 ; Vol. 23, No. 6.
    @article{b3e390f2e1c142b893a43b871ddb241f,
    title = "Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/blaCMY-2 resistance plasmids",
    abstract = "In 2012 and 2014 the Norwegian monitoring programme for antimicrobial resistance in the veterinary and food production sectors (NORM-VET) showed that 124 of a total of 406 samples (31{\%}) of Norwegian retail chicken meat was contaminated with extended-spectrum cephalosporin-resistant Escherichia coli. The aim of this study was to compare selected cephalosporin-resistant E. coli from humans and poultry to determine their genetic relatedness based on whole genome sequencing (WGS). E. coli representing three prevalent cephalosporin-resistant multi-locus sequence types (STs) isolated from poultry (n=17) were selected from the NORM-VET strain collections. All strains carried an IncK plasmid with a blaCMY-2 gene. Clinical E. coli isolates (n=284) with AmpC-mediated resistance were collected at Norwegian microbiology laboratories from 2010 to 2014. PCR screening showed that 29 of the clinical isolates harboured both IncK and blaCMY-2. All IncK/blaCMY-2 positive isolates were analysed by WGS-based bioinformatics tools. Analysis of single nucleotide polymorphisms (SNP) in 2.5 Mbp of shared genome sequences showed close relationship with less than 15 SNP differences between five clinical isolates from urinary tract infections, and the ST38 isolates from poultry. Furthermore, all of the 29 clinical isolates harboured IncK/blaCMY-2 plasmid variants highly similar to the IncK/blaCMY-2 plasmid present in the poultry isolates. Our results provide support for the hypothesis that clonal transfer of cephalosporin-resistant E. coli from chicken meat to humans may occur, and may cause difficult to treat infections. Furthermore, these E. coli can be a source of AmpC resistance plasmids for opportunistic pathogens in the human microbiota.",
    keywords = "Zoonotic transfer of cephalosporin resistant E. coli, Chicken meat, Plasmid-mediated AmpC transmission, Urinary tract infections",
    author = "Berg, {E. S.} and Wester, {A. L.} and Johanne Ahrenfeldt and Mo, {S. S.} and Sletteme{\aa}s, {J. S.} and M Steinbakk and {\O}. Samuelsen and N. Grude and Simonsen, {G. S.} and L{\o}hr, {I. H.} and J{\o}rgensen, {S. B.} and S. Tofteland and Ole Lund and Dahle, {U. R.} and M. Sunde",
    year = "2017",
    doi = "10.1016/j.cmi.2016.12.035",
    language = "English",
    volume = "23",
    journal = "Clinical Microbiology and Infection",
    issn = "1198-743X",
    publisher = "Elsevier",
    number = "6",

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    Berg, ES, Wester, AL, Ahrenfeldt, J, Mo, SS, Slettemeås, JS, Steinbakk, M, Samuelsen, Ø, Grude, N, Simonsen, GS, Løhr, IH, Jørgensen, SB, Tofteland, S, Lund, O, Dahle, UR & Sunde, M 2017, 'Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/blaCMY-2 resistance plasmids', Clinical Microbiology and Infection, vol. 23, no. 6. https://doi.org/10.1016/j.cmi.2016.12.035

    Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/blaCMY-2 resistance plasmids. / Berg, E. S.; Wester, A. L.; Ahrenfeldt, Johanne; Mo, S. S.; Slettemeås, J. S.; Steinbakk, M; Samuelsen, Ø.; Grude, N.; Simonsen, G. S.; Løhr, I. H.; Jørgensen, S. B.; Tofteland, S.; Lund, Ole; Dahle, U. R.; Sunde, M.

    In: Clinical Microbiology and Infection, Vol. 23, No. 6, 2017.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/blaCMY-2 resistance plasmids

    AU - Berg, E. S.

    AU - Wester, A. L.

    AU - Ahrenfeldt, Johanne

    AU - Mo, S. S.

    AU - Slettemeås, J. S.

    AU - Steinbakk, M

    AU - Samuelsen, Ø.

    AU - Grude, N.

    AU - Simonsen, G. S.

    AU - Løhr, I. H.

    AU - Jørgensen, S. B.

    AU - Tofteland, S.

    AU - Lund, Ole

    AU - Dahle, U. R.

    AU - Sunde, M.

    PY - 2017

    Y1 - 2017

    N2 - In 2012 and 2014 the Norwegian monitoring programme for antimicrobial resistance in the veterinary and food production sectors (NORM-VET) showed that 124 of a total of 406 samples (31%) of Norwegian retail chicken meat was contaminated with extended-spectrum cephalosporin-resistant Escherichia coli. The aim of this study was to compare selected cephalosporin-resistant E. coli from humans and poultry to determine their genetic relatedness based on whole genome sequencing (WGS). E. coli representing three prevalent cephalosporin-resistant multi-locus sequence types (STs) isolated from poultry (n=17) were selected from the NORM-VET strain collections. All strains carried an IncK plasmid with a blaCMY-2 gene. Clinical E. coli isolates (n=284) with AmpC-mediated resistance were collected at Norwegian microbiology laboratories from 2010 to 2014. PCR screening showed that 29 of the clinical isolates harboured both IncK and blaCMY-2. All IncK/blaCMY-2 positive isolates were analysed by WGS-based bioinformatics tools. Analysis of single nucleotide polymorphisms (SNP) in 2.5 Mbp of shared genome sequences showed close relationship with less than 15 SNP differences between five clinical isolates from urinary tract infections, and the ST38 isolates from poultry. Furthermore, all of the 29 clinical isolates harboured IncK/blaCMY-2 plasmid variants highly similar to the IncK/blaCMY-2 plasmid present in the poultry isolates. Our results provide support for the hypothesis that clonal transfer of cephalosporin-resistant E. coli from chicken meat to humans may occur, and may cause difficult to treat infections. Furthermore, these E. coli can be a source of AmpC resistance plasmids for opportunistic pathogens in the human microbiota.

    AB - In 2012 and 2014 the Norwegian monitoring programme for antimicrobial resistance in the veterinary and food production sectors (NORM-VET) showed that 124 of a total of 406 samples (31%) of Norwegian retail chicken meat was contaminated with extended-spectrum cephalosporin-resistant Escherichia coli. The aim of this study was to compare selected cephalosporin-resistant E. coli from humans and poultry to determine their genetic relatedness based on whole genome sequencing (WGS). E. coli representing three prevalent cephalosporin-resistant multi-locus sequence types (STs) isolated from poultry (n=17) were selected from the NORM-VET strain collections. All strains carried an IncK plasmid with a blaCMY-2 gene. Clinical E. coli isolates (n=284) with AmpC-mediated resistance were collected at Norwegian microbiology laboratories from 2010 to 2014. PCR screening showed that 29 of the clinical isolates harboured both IncK and blaCMY-2. All IncK/blaCMY-2 positive isolates were analysed by WGS-based bioinformatics tools. Analysis of single nucleotide polymorphisms (SNP) in 2.5 Mbp of shared genome sequences showed close relationship with less than 15 SNP differences between five clinical isolates from urinary tract infections, and the ST38 isolates from poultry. Furthermore, all of the 29 clinical isolates harboured IncK/blaCMY-2 plasmid variants highly similar to the IncK/blaCMY-2 plasmid present in the poultry isolates. Our results provide support for the hypothesis that clonal transfer of cephalosporin-resistant E. coli from chicken meat to humans may occur, and may cause difficult to treat infections. Furthermore, these E. coli can be a source of AmpC resistance plasmids for opportunistic pathogens in the human microbiota.

    KW - Zoonotic transfer of cephalosporin resistant E. coli

    KW - Chicken meat

    KW - Plasmid-mediated AmpC transmission

    KW - Urinary tract infections

    U2 - 10.1016/j.cmi.2016.12.035

    DO - 10.1016/j.cmi.2016.12.035

    M3 - Journal article

    VL - 23

    JO - Clinical Microbiology and Infection

    JF - Clinical Microbiology and Infection

    SN - 1198-743X

    IS - 6

    ER -