Non-invasive in-cell determination of free cytosolic [NAD+]/ [NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes

Caspar Elo Christensen; Magnus Karlsson; Jakob R. Winther; Pernille Rose Jensen; Mathilde Hauge Lerche

doi:10.1074/jbc.M113.498626

Non-invasive in-cell determination of free cytosolic [NAD+]/ [NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes

Caspar Elo Christensen, Magnus Karlsson, Jakob R. Winther, Pernille Rose Jensen, Mathilde Hauge Lerche

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Background: Free cytosolic [NAD+]/[NADH] ratio maintains cellular redox homeostasis and is a cellular metabolic readout. Results: Pyruvate/lactate ratios show distinct metabolic phenotypes and are used to derive free cytosolic [NAD+]/[NADH] ratios. Conclusion: Determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose is applicable to a wide selection of cell types. Significance: This metabolic phenotyping may be a crucial tool to understand pathologies, and to diagnose and measure effects of therapies. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

Original language	English
Journal	Journal of Biological Chemistry
Volume	289
Issue number	4
Pages (from-to)	2344-2352
ISSN	0021-9258
DOIs	https://doi.org/10.1074/jbc.M113.498626
Publication status	Published - 2014
Externally published	Yes

Keywords

Biochemistry
Cell Biology
Molecular Biology
Cell types
Cellular redox
Cytosolic
Hyperpolarized
Metabolic phenotype
Phenotyping
Pyruvates
Wide selection
Glucose
Metabolism
1 carbon lactate c 13
1 carbon pyruvic acid c 13
glucose
nicotinamide adenine dinucleotide
pentose phosphate
radioisotope
reduced nicotinamide adenine dinucleotide
unclassified drug
article
breast cancer
cancer cell
cell kinetics
cell metabolism
controlled study
free cytosolic nicotinamide adenine dinucleotide reduced nicotinamide adenine dinucleotide ratio
glycolysis
homeostasis
human
human cell
hyperpolarization
lactate pyruvate ratio
male
metabolic parameters
metabolite
non invasive procedure
oxidation reduction state
phenotype
priority journal
prostate cancer
Breast Cancer
Hyperpolarization
Metabolic Phenotype
NAD
NADH
Prostate Cancer
Breast Neoplasms
Cell Line, Tumor
Female
Glycolysis
Humans
Lactic Acid
Male
Prostatic Neoplasms
Pyruvic Acid
cellular glycolytic activity
energy metabolism
metabolic alteration
metabolic phenotypic variation
breast cancer Breast Neoplasms (MeSH) neoplastic disease, reproductive system disease/female
prostate cancer Prostatic Neoplasms (MeSH) urologic disease, reproductive system disease/male, neoplastic disease
hyperpolarized glucose
isotopically labeled metabolic bioprobe
lactate 113-21-3
NAD+
NADH 58-68-4
pyruvate 57-60-3
02502, Cytology - General
10060, Biochemistry studies - General
10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
13002, Metabolism - General metabolism and metabolic pathways
15506, Urinary system - Pathology
16506, Reproductive system - Pathology
24004, Neoplasms - Pathology, clinical aspects and systemic effects
magnetic enhancement technique laboratory techniques
non-invasive in-cell determination laboratory techniques, histology and cytology techniques
Biochemistry and Molecular Biophysics
Molecular Biophysics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1074/jbc.M113.498626

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title = "Non-invasive in-cell determination of free cytosolic [NAD+]/ [NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes",

abstract = "Background: Free cytosolic [NAD+]/[NADH] ratio maintains cellular redox homeostasis and is a cellular metabolic readout. Results: Pyruvate/lactate ratios show distinct metabolic phenotypes and are used to derive free cytosolic [NAD+]/[NADH] ratios. Conclusion: Determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose is applicable to a wide selection of cell types. Significance: This metabolic phenotyping may be a crucial tool to understand pathologies, and to diagnose and measure effects of therapies. {\textcopyright} 2014 by The American Society for Biochemistry and Molecular Biology, Inc.",

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author = "Christensen, {Caspar Elo} and Magnus Karlsson and Winther, {Jakob R.} and Jensen, {Pernille Rose} and Lerche, {Mathilde Hauge}",

year = "2014",

doi = "10.1074/jbc.M113.498626",

language = "English",

volume = "289",

pages = "2344--2352",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology, Inc.",

number = "4",

}

Non-invasive in-cell determination of free cytosolic [NAD+]/ [NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes. / Christensen, Caspar Elo; Karlsson, Magnus; Winther, Jakob R. et al.
In: Journal of Biological Chemistry, Vol. 289, No. 4, 2014, p. 2344-2352.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Non-invasive in-cell determination of free cytosolic [NAD+]/ [NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes

AU - Christensen, Caspar Elo

AU - Karlsson, Magnus

AU - Winther, Jakob R.

AU - Jensen, Pernille Rose

AU - Lerche, Mathilde Hauge

PY - 2014

Y1 - 2014

N2 - Background: Free cytosolic [NAD+]/[NADH] ratio maintains cellular redox homeostasis and is a cellular metabolic readout. Results: Pyruvate/lactate ratios show distinct metabolic phenotypes and are used to derive free cytosolic [NAD+]/[NADH] ratios. Conclusion: Determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose is applicable to a wide selection of cell types. Significance: This metabolic phenotyping may be a crucial tool to understand pathologies, and to diagnose and measure effects of therapies. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

AB - Background: Free cytosolic [NAD+]/[NADH] ratio maintains cellular redox homeostasis and is a cellular metabolic readout. Results: Pyruvate/lactate ratios show distinct metabolic phenotypes and are used to derive free cytosolic [NAD+]/[NADH] ratios. Conclusion: Determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose is applicable to a wide selection of cell types. Significance: This metabolic phenotyping may be a crucial tool to understand pathologies, and to diagnose and measure effects of therapies. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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KW - Cell Biology

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KW - Glucose

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KW - 1 carbon lactate c 13

KW - 1 carbon pyruvic acid c 13

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KW - nicotinamide adenine dinucleotide

KW - pentose phosphate

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KW - homeostasis

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KW - human cell

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KW - lactate pyruvate ratio

KW - male

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KW - oxidation reduction state

KW - phenotype

KW - priority journal

KW - prostate cancer

KW - Breast Cancer

KW - Hyperpolarization

KW - Metabolic Phenotype

KW - NAD

KW - NADH

KW - Prostate Cancer

KW - Breast Neoplasms

KW - Cell Line, Tumor

KW - Female

KW - Glycolysis

KW - Humans

KW - Lactic Acid

KW - Male

KW - Prostatic Neoplasms

KW - Pyruvic Acid

KW - cellular glycolytic activity

KW - energy metabolism

KW - metabolic alteration

KW - metabolic phenotypic variation

KW - breast cancer Breast Neoplasms (MeSH) neoplastic disease, reproductive system disease/female

KW - prostate cancer Prostatic Neoplasms (MeSH) urologic disease, reproductive system disease/male, neoplastic disease

KW - hyperpolarized glucose

KW - isotopically labeled metabolic bioprobe

KW - lactate 113-21-3

KW - NAD+

KW - NADH 58-68-4

KW - pyruvate 57-60-3

KW - 02502, Cytology - General

KW - 10060, Biochemistry studies - General

KW - 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines

KW - 13002, Metabolism - General metabolism and metabolic pathways

KW - 15506, Urinary system - Pathology

KW - 16506, Reproductive system - Pathology

KW - 24004, Neoplasms - Pathology, clinical aspects and systemic effects

KW - magnetic enhancement technique laboratory techniques

KW - non-invasive in-cell determination laboratory techniques, histology and cytology techniques

KW - Biochemistry and Molecular Biophysics

KW - Molecular Biophysics

U2 - 10.1074/jbc.M113.498626

DO - 10.1074/jbc.M113.498626

M3 - Journal article

SN - 0021-9258

VL - 289

SP - 2344

EP - 2352

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 4

ER -

Non-invasive in-cell determination of free cytosolic [NAD+]/ [NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes

Abstract

Keywords

UN SDGs

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