Non-cytotoxic polymer vesicles for rapid and efficient intracellular delivery

Hannah Lomas, Marzia Massignani, Khairuddin A. Abdullah, Irene Canton, Caterina Lo Presti, Sheila MacNeil, Jianzhong Du, Adam Blanazs, Peter Jeppe Madsen, Steven P. Armes, Andrew L. Lewis, Giuseppe Battaglia

Research output: Contribution to journalJournal articleResearchpeer-review


We have recently achieved efficient cytosolic delivery by using pH-sensitive poly(2-(methacryloyloxy) ethylphosphorylcholine)-co-poly(2-( diisopropylamino)ethylmethacrylate) (PMPC-PDPA) diblock copolymers that self-assemble to form vesicles, known as polymersomes, in aqueous solution. It is particularly noteworthy that these diblock copolymers form stable polymersomes at physiological pH but rapidly dissociate below pH 6 to give molecularly-dissolved copolymer chains (unimers). These PMPC-PDPA polymersomes are used to encapsulate nucleic acids for efficient intracellular delivery. Confocal laser scanning microscopy and fluorescence flow cytometry are used to quantify cellular uptake and to study the kinetics of this process. Finally, we examine how PMPC-PDPA polymersomes affect the viability of primary human cells (human dermal fibroblasts (HDF)), paying particular regard to whether inflammatory responses are triggered.
Original languageEnglish
JournalFaraday Discussions
Pages (from-to)143-159
Number of pages17
Publication statusPublished - 2008
Externally publishedYes


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