No priming of the immune response in newborn Brown Norway rats dosed with ovalbumin in the mouth

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Background: Other researchers have reported that the specific immune response to subsequent antigen challenge is primed in newborn mice or rats dosed orally by gavage. We wanted to investigate if priming of a subsequent specific IgE response could be achieved by dosing newborn rats orally with ovalbumin and if this method could be used in an animal model for food allergy. Methods: Newborn Brown Norway rats were dosed with ovalbumin in the mouth (100 mug or 6 mg). As young adults, the animals were dosed by gavage for 35 days with 1 mg ovalbumin/day or once intraperitoneally with 100 mug. Control groups were dosed by gavage or intraperitoneally but not as neonates. Additionally, young adult rats were dosed with 1 mg ovalbumin/day in the mouth for 35 days. Sera from individual animals were analysed for specific IgE and specific IgG. Results: In all experiments with neonatal rats the specific IgE and IgG responses were decreased compared to the control groups, however, not always reaching statistical significance. A statistical significant decrease in the specific immune response was found in young adult rats dosed in the mouth as compared to by gavage. Conclusions: Dosing Brown Norway rats with ovalbumin in the mouth as neonates do not prime the specific immune response. The decrease in immune response found in our experiments when dosing newborn animals in the mouth in opposition to the priming seen by others when dosing by intra-gastric intubation may be explained by a dissimilar antigen presentation when dosing includes both oral mucosa and gut. Copyright (C) 2003 S. Karger AG, Basel.
Original languageEnglish
JournalINTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
Volume130
Issue number1
Pages (from-to)66-72
ISSN1018-2438
DOIs
Publication statusPublished - 2003

Cite this

@article{8e477d2d59bb43d1bc0ba9b58be22566,
title = "No priming of the immune response in newborn Brown Norway rats dosed with ovalbumin in the mouth",
abstract = "Background: Other researchers have reported that the specific immune response to subsequent antigen challenge is primed in newborn mice or rats dosed orally by gavage. We wanted to investigate if priming of a subsequent specific IgE response could be achieved by dosing newborn rats orally with ovalbumin and if this method could be used in an animal model for food allergy. Methods: Newborn Brown Norway rats were dosed with ovalbumin in the mouth (100 mug or 6 mg). As young adults, the animals were dosed by gavage for 35 days with 1 mg ovalbumin/day or once intraperitoneally with 100 mug. Control groups were dosed by gavage or intraperitoneally but not as neonates. Additionally, young adult rats were dosed with 1 mg ovalbumin/day in the mouth for 35 days. Sera from individual animals were analysed for specific IgE and specific IgG. Results: In all experiments with neonatal rats the specific IgE and IgG responses were decreased compared to the control groups, however, not always reaching statistical significance. A statistical significant decrease in the specific immune response was found in young adult rats dosed in the mouth as compared to by gavage. Conclusions: Dosing Brown Norway rats with ovalbumin in the mouth as neonates do not prime the specific immune response. The decrease in immune response found in our experiments when dosing newborn animals in the mouth in opposition to the priming seen by others when dosing by intra-gastric intubation may be explained by a dissimilar antigen presentation when dosing includes both oral mucosa and gut. Copyright (C) 2003 S. Karger AG, Basel.",
author = "Madsen, {Charlotte Bernhard} and Kirsten Pilegaard",
year = "2003",
doi = "10.1159/000068368",
language = "English",
volume = "130",
pages = "66--72",
journal = "International Archives of Allergy and Immunology",
issn = "1018-2438",
publisher = "S. Karger AG",
number = "1",

}

No priming of the immune response in newborn Brown Norway rats dosed with ovalbumin in the mouth. / Madsen, Charlotte Bernhard; Pilegaard, Kirsten.

In: INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, Vol. 130, No. 1, 2003, p. 66-72.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - No priming of the immune response in newborn Brown Norway rats dosed with ovalbumin in the mouth

AU - Madsen, Charlotte Bernhard

AU - Pilegaard, Kirsten

PY - 2003

Y1 - 2003

N2 - Background: Other researchers have reported that the specific immune response to subsequent antigen challenge is primed in newborn mice or rats dosed orally by gavage. We wanted to investigate if priming of a subsequent specific IgE response could be achieved by dosing newborn rats orally with ovalbumin and if this method could be used in an animal model for food allergy. Methods: Newborn Brown Norway rats were dosed with ovalbumin in the mouth (100 mug or 6 mg). As young adults, the animals were dosed by gavage for 35 days with 1 mg ovalbumin/day or once intraperitoneally with 100 mug. Control groups were dosed by gavage or intraperitoneally but not as neonates. Additionally, young adult rats were dosed with 1 mg ovalbumin/day in the mouth for 35 days. Sera from individual animals were analysed for specific IgE and specific IgG. Results: In all experiments with neonatal rats the specific IgE and IgG responses were decreased compared to the control groups, however, not always reaching statistical significance. A statistical significant decrease in the specific immune response was found in young adult rats dosed in the mouth as compared to by gavage. Conclusions: Dosing Brown Norway rats with ovalbumin in the mouth as neonates do not prime the specific immune response. The decrease in immune response found in our experiments when dosing newborn animals in the mouth in opposition to the priming seen by others when dosing by intra-gastric intubation may be explained by a dissimilar antigen presentation when dosing includes both oral mucosa and gut. Copyright (C) 2003 S. Karger AG, Basel.

AB - Background: Other researchers have reported that the specific immune response to subsequent antigen challenge is primed in newborn mice or rats dosed orally by gavage. We wanted to investigate if priming of a subsequent specific IgE response could be achieved by dosing newborn rats orally with ovalbumin and if this method could be used in an animal model for food allergy. Methods: Newborn Brown Norway rats were dosed with ovalbumin in the mouth (100 mug or 6 mg). As young adults, the animals were dosed by gavage for 35 days with 1 mg ovalbumin/day or once intraperitoneally with 100 mug. Control groups were dosed by gavage or intraperitoneally but not as neonates. Additionally, young adult rats were dosed with 1 mg ovalbumin/day in the mouth for 35 days. Sera from individual animals were analysed for specific IgE and specific IgG. Results: In all experiments with neonatal rats the specific IgE and IgG responses were decreased compared to the control groups, however, not always reaching statistical significance. A statistical significant decrease in the specific immune response was found in young adult rats dosed in the mouth as compared to by gavage. Conclusions: Dosing Brown Norway rats with ovalbumin in the mouth as neonates do not prime the specific immune response. The decrease in immune response found in our experiments when dosing newborn animals in the mouth in opposition to the priming seen by others when dosing by intra-gastric intubation may be explained by a dissimilar antigen presentation when dosing includes both oral mucosa and gut. Copyright (C) 2003 S. Karger AG, Basel.

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VL - 130

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JO - International Archives of Allergy and Immunology

JF - International Archives of Allergy and Immunology

SN - 1018-2438

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