No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs

Nicole Bakkegård Goecke, Charlotte Kristiane Hjulsager, H. Kongsted, Mette Boye, S. Rasmussen, F. Granberg, T K Fischer, S E Midgley, Lasse Dam Rasmussen, Ø. Angen, Jytte Pihl Nielsen, Sven Erik Lind Jorsal, L. E. Larsen

Research output: Contribution to journalJournal articleResearchpeer-review

264 Downloads (Pure)

Abstract

The aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology. Four well-managed herds experiencing neonatal diarrhoea and suspected to be affected by NNPDS were included in a case-control set up. A total of 989 piglets were clinically examined on a daily basis. Samples from diarrhoeic and non-diarrhoeic piglets at the age of three to seven days were selected for extensive virological examination using specific real time polymerase chain reactions (qPCRs) and general virus detection methods. A total of 91.7% of the animals tested positive by reverse transcription qPCR (RT-qPCR) for porcine kobuvirus 1 (PKV-1) while 9% and 3% were found to be positive for rotavirus A and porcine teschovirus (PTV), respectively. The overall prevalence of porcine astrovirus (PAstV) was 75% with 69.8% of the PAstV positive pigs infected with PAstV type 3. No animals tested positive for rotavirus C, coronavirus (TGEV, PEDV and PRCV), sapovirus, enterovirus, parechovirus, saffoldvirus, cosavirus, klassevirus or porcine circovirus type 2 (PCV2). Microarray analyses performed on a total of 18 animals were all negative, as were eight animals examined by Transmission Electron Microscopy (TEM). Using Next Generation de novo sequencing (de novo NGS) on pools of samples from case animals within all herds, PKV-1 was detected in four herds and rotavirus A, rotavirus C and PTV were detected in one herd each. Our detailed analyses of piglets from NNPDS-affected herds demonstrated that viruses did not pose a significant contribution to NNPDS. However, further investigations are needed to investigate if a systemic virus infection plays a role in the pathogenesis of NNPDS.
Original languageEnglish
Article number315
JournalB M C Veterinary Research
Volume13
Issue number1
Number of pages11
ISSN1746-6148
DOIs
Publication statusPublished - 2017

Keywords

  • Diarrhoea
  • NNPDS
  • Neonatal piglets
  • Virus

Cite this

Goecke, Nicole Bakkegård ; Hjulsager, Charlotte Kristiane ; Kongsted, H. ; Boye, Mette ; Rasmussen, S. ; Granberg, F. ; Fischer, T K ; Midgley, S E ; Rasmussen, Lasse Dam ; Angen, Ø. ; Nielsen, Jytte Pihl ; Jorsal, Sven Erik Lind ; Larsen, L. E. / No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs. In: B M C Veterinary Research. 2017 ; Vol. 13, No. 1.
@article{56669d8e1d274a65a9c12ef9afe24bf0,
title = "No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs",
abstract = "The aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology. Four well-managed herds experiencing neonatal diarrhoea and suspected to be affected by NNPDS were included in a case-control set up. A total of 989 piglets were clinically examined on a daily basis. Samples from diarrhoeic and non-diarrhoeic piglets at the age of three to seven days were selected for extensive virological examination using specific real time polymerase chain reactions (qPCRs) and general virus detection methods. A total of 91.7{\%} of the animals tested positive by reverse transcription qPCR (RT-qPCR) for porcine kobuvirus 1 (PKV-1) while 9{\%} and 3{\%} were found to be positive for rotavirus A and porcine teschovirus (PTV), respectively. The overall prevalence of porcine astrovirus (PAstV) was 75{\%} with 69.8{\%} of the PAstV positive pigs infected with PAstV type 3. No animals tested positive for rotavirus C, coronavirus (TGEV, PEDV and PRCV), sapovirus, enterovirus, parechovirus, saffoldvirus, cosavirus, klassevirus or porcine circovirus type 2 (PCV2). Microarray analyses performed on a total of 18 animals were all negative, as were eight animals examined by Transmission Electron Microscopy (TEM). Using Next Generation de novo sequencing (de novo NGS) on pools of samples from case animals within all herds, PKV-1 was detected in four herds and rotavirus A, rotavirus C and PTV were detected in one herd each. Our detailed analyses of piglets from NNPDS-affected herds demonstrated that viruses did not pose a significant contribution to NNPDS. However, further investigations are needed to investigate if a systemic virus infection plays a role in the pathogenesis of NNPDS.",
keywords = "Diarrhoea, NNPDS, Neonatal piglets, Virus",
author = "Goecke, {Nicole Bakkeg{\aa}rd} and Hjulsager, {Charlotte Kristiane} and H. Kongsted and Mette Boye and S. Rasmussen and F. Granberg and Fischer, {T K} and Midgley, {S E} and Rasmussen, {Lasse Dam} and {\O}. Angen and Nielsen, {Jytte Pihl} and Jorsal, {Sven Erik Lind} and Larsen, {L. E.}",
year = "2017",
doi = "10.1186/s12917-017-1239-5",
language = "English",
volume = "13",
journal = "B M C Veterinary Research",
issn = "1746-6148",
publisher = "BioMed Central Ltd.",
number = "1",

}

Goecke, NB, Hjulsager, CK, Kongsted, H, Boye, M, Rasmussen, S, Granberg, F, Fischer, TK, Midgley, SE, Rasmussen, LD, Angen, Ø, Nielsen, JP, Jorsal, SEL & Larsen, LE 2017, 'No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs', B M C Veterinary Research, vol. 13, no. 1, 315. https://doi.org/10.1186/s12917-017-1239-5

No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs. / Goecke, Nicole Bakkegård; Hjulsager, Charlotte Kristiane; Kongsted, H.; Boye, Mette; Rasmussen, S.; Granberg, F.; Fischer, T K; Midgley, S E; Rasmussen, Lasse Dam; Angen, Ø.; Nielsen, Jytte Pihl; Jorsal, Sven Erik Lind; Larsen, L. E.

In: B M C Veterinary Research, Vol. 13, No. 1, 315, 2017.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs

AU - Goecke, Nicole Bakkegård

AU - Hjulsager, Charlotte Kristiane

AU - Kongsted, H.

AU - Boye, Mette

AU - Rasmussen, S.

AU - Granberg, F.

AU - Fischer, T K

AU - Midgley, S E

AU - Rasmussen, Lasse Dam

AU - Angen, Ø.

AU - Nielsen, Jytte Pihl

AU - Jorsal, Sven Erik Lind

AU - Larsen, L. E.

PY - 2017

Y1 - 2017

N2 - The aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology. Four well-managed herds experiencing neonatal diarrhoea and suspected to be affected by NNPDS were included in a case-control set up. A total of 989 piglets were clinically examined on a daily basis. Samples from diarrhoeic and non-diarrhoeic piglets at the age of three to seven days were selected for extensive virological examination using specific real time polymerase chain reactions (qPCRs) and general virus detection methods. A total of 91.7% of the animals tested positive by reverse transcription qPCR (RT-qPCR) for porcine kobuvirus 1 (PKV-1) while 9% and 3% were found to be positive for rotavirus A and porcine teschovirus (PTV), respectively. The overall prevalence of porcine astrovirus (PAstV) was 75% with 69.8% of the PAstV positive pigs infected with PAstV type 3. No animals tested positive for rotavirus C, coronavirus (TGEV, PEDV and PRCV), sapovirus, enterovirus, parechovirus, saffoldvirus, cosavirus, klassevirus or porcine circovirus type 2 (PCV2). Microarray analyses performed on a total of 18 animals were all negative, as were eight animals examined by Transmission Electron Microscopy (TEM). Using Next Generation de novo sequencing (de novo NGS) on pools of samples from case animals within all herds, PKV-1 was detected in four herds and rotavirus A, rotavirus C and PTV were detected in one herd each. Our detailed analyses of piglets from NNPDS-affected herds demonstrated that viruses did not pose a significant contribution to NNPDS. However, further investigations are needed to investigate if a systemic virus infection plays a role in the pathogenesis of NNPDS.

AB - The aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology. Four well-managed herds experiencing neonatal diarrhoea and suspected to be affected by NNPDS were included in a case-control set up. A total of 989 piglets were clinically examined on a daily basis. Samples from diarrhoeic and non-diarrhoeic piglets at the age of three to seven days were selected for extensive virological examination using specific real time polymerase chain reactions (qPCRs) and general virus detection methods. A total of 91.7% of the animals tested positive by reverse transcription qPCR (RT-qPCR) for porcine kobuvirus 1 (PKV-1) while 9% and 3% were found to be positive for rotavirus A and porcine teschovirus (PTV), respectively. The overall prevalence of porcine astrovirus (PAstV) was 75% with 69.8% of the PAstV positive pigs infected with PAstV type 3. No animals tested positive for rotavirus C, coronavirus (TGEV, PEDV and PRCV), sapovirus, enterovirus, parechovirus, saffoldvirus, cosavirus, klassevirus or porcine circovirus type 2 (PCV2). Microarray analyses performed on a total of 18 animals were all negative, as were eight animals examined by Transmission Electron Microscopy (TEM). Using Next Generation de novo sequencing (de novo NGS) on pools of samples from case animals within all herds, PKV-1 was detected in four herds and rotavirus A, rotavirus C and PTV were detected in one herd each. Our detailed analyses of piglets from NNPDS-affected herds demonstrated that viruses did not pose a significant contribution to NNPDS. However, further investigations are needed to investigate if a systemic virus infection plays a role in the pathogenesis of NNPDS.

KW - Diarrhoea

KW - NNPDS

KW - Neonatal piglets

KW - Virus

U2 - 10.1186/s12917-017-1239-5

DO - 10.1186/s12917-017-1239-5

M3 - Journal article

VL - 13

JO - B M C Veterinary Research

JF - B M C Veterinary Research

SN - 1746-6148

IS - 1

M1 - 315

ER -