No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs

Nicole Bakkegård Goecke, Charlotte Kristiane Hjulsager, H. Kongsted, Mette Boye, S. Rasmussen, F. Granberg, T K Fischer, S E Midgley, Lasse Dam Rasmussen, Ø. Angen, Jytte Pihl Nielsen, Sven Erik Lind Jorsal, L. E. Larsen

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    Abstract

    The aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology. Four well-managed herds experiencing neonatal diarrhoea and suspected to be affected by NNPDS were included in a case-control set up. A total of 989 piglets were clinically examined on a daily basis. Samples from diarrhoeic and non-diarrhoeic piglets at the age of three to seven days were selected for extensive virological examination using specific real time polymerase chain reactions (qPCRs) and general virus detection methods. A total of 91.7% of the animals tested positive by reverse transcription qPCR (RT-qPCR) for porcine kobuvirus 1 (PKV-1) while 9% and 3% were found to be positive for rotavirus A and porcine teschovirus (PTV), respectively. The overall prevalence of porcine astrovirus (PAstV) was 75% with 69.8% of the PAstV positive pigs infected with PAstV type 3. No animals tested positive for rotavirus C, coronavirus (TGEV, PEDV and PRCV), sapovirus, enterovirus, parechovirus, saffoldvirus, cosavirus, klassevirus or porcine circovirus type 2 (PCV2). Microarray analyses performed on a total of 18 animals were all negative, as were eight animals examined by Transmission Electron Microscopy (TEM). Using Next Generation de novo sequencing (de novo NGS) on pools of samples from case animals within all herds, PKV-1 was detected in four herds and rotavirus A, rotavirus C and PTV were detected in one herd each. Our detailed analyses of piglets from NNPDS-affected herds demonstrated that viruses did not pose a significant contribution to NNPDS. However, further investigations are needed to investigate if a systemic virus infection plays a role in the pathogenesis of NNPDS.
    Original languageEnglish
    Article number315
    JournalB M C Veterinary Research
    Volume13
    Issue number1
    Number of pages11
    ISSN1746-6148
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Diarrhoea
    • NNPDS
    • Neonatal piglets
    • Virus

    Cite this

    Goecke, Nicole Bakkegård ; Hjulsager, Charlotte Kristiane ; Kongsted, H. ; Boye, Mette ; Rasmussen, S. ; Granberg, F. ; Fischer, T K ; Midgley, S E ; Rasmussen, Lasse Dam ; Angen, Ø. ; Nielsen, Jytte Pihl ; Jorsal, Sven Erik Lind ; Larsen, L. E. / No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs. In: B M C Veterinary Research. 2017 ; Vol. 13, No. 1.
    @article{56669d8e1d274a65a9c12ef9afe24bf0,
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    abstract = "The aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology. Four well-managed herds experiencing neonatal diarrhoea and suspected to be affected by NNPDS were included in a case-control set up. A total of 989 piglets were clinically examined on a daily basis. Samples from diarrhoeic and non-diarrhoeic piglets at the age of three to seven days were selected for extensive virological examination using specific real time polymerase chain reactions (qPCRs) and general virus detection methods. A total of 91.7{\%} of the animals tested positive by reverse transcription qPCR (RT-qPCR) for porcine kobuvirus 1 (PKV-1) while 9{\%} and 3{\%} were found to be positive for rotavirus A and porcine teschovirus (PTV), respectively. The overall prevalence of porcine astrovirus (PAstV) was 75{\%} with 69.8{\%} of the PAstV positive pigs infected with PAstV type 3. No animals tested positive for rotavirus C, coronavirus (TGEV, PEDV and PRCV), sapovirus, enterovirus, parechovirus, saffoldvirus, cosavirus, klassevirus or porcine circovirus type 2 (PCV2). Microarray analyses performed on a total of 18 animals were all negative, as were eight animals examined by Transmission Electron Microscopy (TEM). Using Next Generation de novo sequencing (de novo NGS) on pools of samples from case animals within all herds, PKV-1 was detected in four herds and rotavirus A, rotavirus C and PTV were detected in one herd each. Our detailed analyses of piglets from NNPDS-affected herds demonstrated that viruses did not pose a significant contribution to NNPDS. However, further investigations are needed to investigate if a systemic virus infection plays a role in the pathogenesis of NNPDS.",
    keywords = "Diarrhoea, NNPDS, Neonatal piglets, Virus",
    author = "Goecke, {Nicole Bakkeg{\aa}rd} and Hjulsager, {Charlotte Kristiane} and H. Kongsted and Mette Boye and S. Rasmussen and F. Granberg and Fischer, {T K} and Midgley, {S E} and Rasmussen, {Lasse Dam} and {\O}. Angen and Nielsen, {Jytte Pihl} and Jorsal, {Sven Erik Lind} and Larsen, {L. E.}",
    year = "2017",
    doi = "10.1186/s12917-017-1239-5",
    language = "English",
    volume = "13",
    journal = "B M C Veterinary Research",
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    publisher = "BioMed Central Ltd.",
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    Goecke, NB, Hjulsager, CK, Kongsted, H, Boye, M, Rasmussen, S, Granberg, F, Fischer, TK, Midgley, SE, Rasmussen, LD, Angen, Ø, Nielsen, JP, Jorsal, SEL & Larsen, LE 2017, 'No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs', B M C Veterinary Research, vol. 13, no. 1, 315. https://doi.org/10.1186/s12917-017-1239-5

    No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs. / Goecke, Nicole Bakkegård; Hjulsager, Charlotte Kristiane; Kongsted, H.; Boye, Mette; Rasmussen, S.; Granberg, F.; Fischer, T K; Midgley, S E; Rasmussen, Lasse Dam; Angen, Ø.; Nielsen, Jytte Pihl; Jorsal, Sven Erik Lind; Larsen, L. E.

    In: B M C Veterinary Research, Vol. 13, No. 1, 315, 2017.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs

    AU - Goecke, Nicole Bakkegård

    AU - Hjulsager, Charlotte Kristiane

    AU - Kongsted, H.

    AU - Boye, Mette

    AU - Rasmussen, S.

    AU - Granberg, F.

    AU - Fischer, T K

    AU - Midgley, S E

    AU - Rasmussen, Lasse Dam

    AU - Angen, Ø.

    AU - Nielsen, Jytte Pihl

    AU - Jorsal, Sven Erik Lind

    AU - Larsen, L. E.

    PY - 2017

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    N2 - The aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology. Four well-managed herds experiencing neonatal diarrhoea and suspected to be affected by NNPDS were included in a case-control set up. A total of 989 piglets were clinically examined on a daily basis. Samples from diarrhoeic and non-diarrhoeic piglets at the age of three to seven days were selected for extensive virological examination using specific real time polymerase chain reactions (qPCRs) and general virus detection methods. A total of 91.7% of the animals tested positive by reverse transcription qPCR (RT-qPCR) for porcine kobuvirus 1 (PKV-1) while 9% and 3% were found to be positive for rotavirus A and porcine teschovirus (PTV), respectively. The overall prevalence of porcine astrovirus (PAstV) was 75% with 69.8% of the PAstV positive pigs infected with PAstV type 3. No animals tested positive for rotavirus C, coronavirus (TGEV, PEDV and PRCV), sapovirus, enterovirus, parechovirus, saffoldvirus, cosavirus, klassevirus or porcine circovirus type 2 (PCV2). Microarray analyses performed on a total of 18 animals were all negative, as were eight animals examined by Transmission Electron Microscopy (TEM). Using Next Generation de novo sequencing (de novo NGS) on pools of samples from case animals within all herds, PKV-1 was detected in four herds and rotavirus A, rotavirus C and PTV were detected in one herd each. Our detailed analyses of piglets from NNPDS-affected herds demonstrated that viruses did not pose a significant contribution to NNPDS. However, further investigations are needed to investigate if a systemic virus infection plays a role in the pathogenesis of NNPDS.

    AB - The aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology. Four well-managed herds experiencing neonatal diarrhoea and suspected to be affected by NNPDS were included in a case-control set up. A total of 989 piglets were clinically examined on a daily basis. Samples from diarrhoeic and non-diarrhoeic piglets at the age of three to seven days were selected for extensive virological examination using specific real time polymerase chain reactions (qPCRs) and general virus detection methods. A total of 91.7% of the animals tested positive by reverse transcription qPCR (RT-qPCR) for porcine kobuvirus 1 (PKV-1) while 9% and 3% were found to be positive for rotavirus A and porcine teschovirus (PTV), respectively. The overall prevalence of porcine astrovirus (PAstV) was 75% with 69.8% of the PAstV positive pigs infected with PAstV type 3. No animals tested positive for rotavirus C, coronavirus (TGEV, PEDV and PRCV), sapovirus, enterovirus, parechovirus, saffoldvirus, cosavirus, klassevirus or porcine circovirus type 2 (PCV2). Microarray analyses performed on a total of 18 animals were all negative, as were eight animals examined by Transmission Electron Microscopy (TEM). Using Next Generation de novo sequencing (de novo NGS) on pools of samples from case animals within all herds, PKV-1 was detected in four herds and rotavirus A, rotavirus C and PTV were detected in one herd each. Our detailed analyses of piglets from NNPDS-affected herds demonstrated that viruses did not pose a significant contribution to NNPDS. However, further investigations are needed to investigate if a systemic virus infection plays a role in the pathogenesis of NNPDS.

    KW - Diarrhoea

    KW - NNPDS

    KW - Neonatal piglets

    KW - Virus

    U2 - 10.1186/s12917-017-1239-5

    DO - 10.1186/s12917-017-1239-5

    M3 - Journal article

    VL - 13

    JO - B M C Veterinary Research

    JF - B M C Veterinary Research

    SN - 1746-6148

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    M1 - 315

    ER -