NMR and MS evidences for a random assembled O-specific chain structure in the LPS of the bacterium Xanthomonas campestris pv. Vitians - A case of unsystematic biosynthetic polymerization

A. Molinaro, C. De Castro, R. Lanzetta, M. Parrilli, Bent O. Petersen, A. Broberg, Jens Øllgaard Duus

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Xanthomonas campestris pv. vitians is a Gram-negative plant-associated bacterium that acts as causative agent of bacterial leaf spot and headrot in lettuce. The lipopolysaccharide of this bacterium is suspected to be an important molecule for adhesion to and infection of the plants. The lipopolysaccharide has been isolated from the phenol phase and the O-specific chain characterized by compositional analysis, high field NMR and MALDI-TOF MS. It consists of a nonrepetitive branched polysaccharide with a rhamnan backbone to which Fuc3NAc is linked. The NMR and MS approach led to the characterization of the fine structure of the polymer, which is randomly assembled. The rhamnan backbone is built up of beta-Rhap and alpha-Rhap, this last is present in one, two or three adjacent units and branched by an alpha-Fucp3NAc unit. This is a real case of a random constituted O-specific chain, therefore biosynthetic studies towards the comprehension of this irregular biosynthesis are needed.
Original languageEnglish
JournalEuropean Journal of Biochemistry
Volume269
Issue number17
Pages (from-to)4185-4193
Number of pages9
ISSN0014-2956
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • Carbohydrate Conformation
  • Lipopolysaccharides
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • O Antigens
  • Phenol
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Xanthomonas campestris
  • 339NCG44TV Phenol
  • CHEMICAL-SHIFTS
  • LIPOPOLYSACCHARIDE
  • POLYSACCHARIDE
  • SPECTRA
  • lipopolysaccharide
  • phytopathogen
  • O-specific chain random polymerization
  • unsystematic biosynthetic polymerization
  • Gram-Negative Aerobic Rods and Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Pseudomonadaceae [06508] Xanthomonas campestris pathogen
  • MALDI-TOF MS MALDI-TOF mass spectrometry analytical method
  • 10060, Biochemistry studies - General
  • 10066, Biochemistry studies - Lipids
  • 10068, Biochemistry studies - Carbohydrates
  • 31000, Physiology and biochemistry of bacteria
  • lipopolysaccharide random assembled O-specific chain structure
  • NMR spectroscopy Imaging Techniques, Spectrum Analysis Techniques, analytical method
  • Biochemistry and Molecular Biophysics
  • Infection
  • Methods and Techniques

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