New system for higher recovery rate of water borne Cryptosporidium oocysts and Giardia cysts

Mohammad Nafi Solaiman Al-Sabi, Jens Gad, Mette Klinting, Claus Mogensen, Jørgen Kurtzhals, Heidi L. Enemark

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

    Abstract

    Background:
    The two most common water borne pathogenic protozoa, Cryptosporidium and Giardia, cause diarrhea worldwide. Detecting these parasites in water samples depends on effective parasite recovery from the water matrix. The reported low recovery rates of the currently used filter methods motivate the development of systems with higher recovery rates.
    Materials and methods:
    Five replicates of IMS purified Cryptosporidium oocysts and Giardia cysts (N=2x103) were injected into a specially coated filter unit with a carefully chosen pore size. Following filtration, sonication was performed at predetermined time intervals to loosen all parasites and other particles from the filter and break up clusters. The concentrated parasite suspension was backwashed and counted after immuno-fluorescence staining.
    Results and discussion:
    Without sonication the recovery rates of both Cryptosporidium and Giardia were <10%. However, recovery rates >85% were recorded when the filter was sonicated. Sonication usually affects parasite viability but could be tuned into a useful tool for enhanced backwash collection of parasites using a specially constructed filter unit and a sonication protocol. The filtration chamber further facilitated the concentration of parasites by ensuring a backwash volume of less than 1ml. The presented design of the filter system can be used as a cheap and
    time effective method of isolating water borne parasites in the laboratory.
    Original languageEnglish
    Title of host publicationIV International Giardia & Cryptosporidium Conference : Conference Abstracts on CD
    Publication date2012
    Pages99
    Publication statusPublished - 2012
    EventIV International Giardia & Cryptosporidium Conference - Wellington, New Zealand
    Duration: 31 Jan 20123 Feb 2012
    http://www.cryptogiardia2012.org/

    Conference

    ConferenceIV International Giardia & Cryptosporidium Conference
    CountryNew Zealand
    CityWellington
    Period31/01/201203/02/2012
    Internet address

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