New producers and new 11-, 19-, and 20-residue peptaibiotics: suzukacillins B and C

C. R. Roehrich, Anita Iversen, T. Degenkolb, W. M. Jaklitsch, H. Voglmayr, Ulf Thrane, A. Vilcinskas, Kristian Fog Nielsen, H. Brueckner

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

    Abstract

    In the course of a project aimed to assess the significance of antibiotics for the producing organism(s) in the natural habitat, we screened a specimen of the fungicolous fungus Hypocrea phellinicola growing on its natural host Phellinus ferruginosus1. Using a peptaibiomics approach2,3, we detected 19- and 20-residue peptide sequences by (U)HPLC/HR-ESI-QqTOF-MS. Structures of peptaibiotics
    found were independently confirmed by analyzing the peptaibiome of an agar plate culture of H. phellinicola CBS 119283 (ex-type) grown under laboratory conditions. Notably, H. phellinicola could be identified as a potent producer of 18-, 19-, (culture) and 20-residue (specimen) peptaibiotics of the suzukacillin-type4. Minor components of the 19-residue peptaibols, herein named suzukacillins C, are assumed to carry a C-terminal residue tentatively assigned as tyrosinol (Tyrol). In addition, the previously isolated suzukacillin B4 was sequenced and shown to be a microheterogeneous mixture of 11-residue peptaibols.
    Original languageEnglish
    JournalJournal of Peptide Science
    Volume18
    Issue numberS1
    Pages (from-to)S73-S73
    ISSN1075-2617
    Publication statusPublished - 2012
    Event32nd EPS Meeting - Athens, Greece
    Duration: 2 Sep 20127 Sep 2012

    Conference

    Conference32nd EPS Meeting
    CountryGreece
    CityAthens
    Period02/09/201207/09/2012

    Bibliographical note

    Poster presentation

    Keywords

    • BIOCHEMISTRY
    • CHEMISTRY,

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