New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

Research output: Contribution to journalJournal article – Annual report year: 2015Researchpeer-review

DOI

  • Author: Wales, Nathan

    University of Copenhagen, Denmark

  • Author: Carøe, Christian

    Metagenomics, Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Kemitorvet, 2800, Lyngby, Denmark

  • Author: Sandoval-Velasco, Marcela

    University of Copenhagen, Denmark

  • Author: Gamba, Cristina

    University of Copenhagen, Denmark

  • Author: Barnett, Ross

    University of Copenhagen, Denmark

  • Author: Samaniego, Jose Alfredo

    University of Copenhagen, Denmark

  • Author: Madrigal, Jazmin Ramos

    University of Copenhagen, Denmark

  • Author: Orlando, Ludovic

    University of Copenhagen, Denmark

  • Author: Gilbert, M. Thomas P.

    University of Copenhagen, Denmark

View graph of relations

An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained
Original languageEnglish
JournalBiotechniques
Volume59
Issue number6
Pages (from-to)368-371
Number of pages4
ISSN0736-6205
DOIs
Publication statusPublished - 2015
CitationsWeb of Science® Times Cited: No match on DOI

ID: 119555966