New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

Nathan Wales; Christian Carøe; Marcela Sandoval-Velasco; Cristina Gamba; Ross Barnett; Jose Alfredo Samaniego; Jazmin Ramos Madrigal; Ludovic Orlando; Thomas P. Gilbert

doi:10.2144/000114364

New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

Nathan Wales
, Christian Carøe
, Marcela Sandoval-Velasco
, Cristina Gamba
, Ross Barnett
, Jose Alfredo Samaniego
, Jazmin Ramos Madrigal
, Ludovic Orlando
, Thomas P. Gilbert

University of Copenhagen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained

Original language	English
Journal	BioTechniques
Volume	59
Issue number	6
Pages (from-to)	368-371
Number of pages	4
ISSN	0736-6205
DOIs	https://doi.org/10.2144/000114364
Publication status	Published - 2015

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title = "New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA",

abstract = "An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained ",

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AU - Wales, Nathan

AU - Carøe, Christian

AU - Sandoval-Velasco, Marcela

AU - Gamba, Cristina

AU - Barnett, Ross

AU - Samaniego, Jose Alfredo

AU - Madrigal, Jazmin Ramos

AU - Orlando, Ludovic

AU - Gilbert, Thomas P.

PY - 2015

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AB - An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained

U2 - 10.2144/000114364

DO - 10.2144/000114364

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SN - 0736-6205

VL - 59

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JO - BioTechniques

JF - BioTechniques

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New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

Abstract

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