New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

Nathan Wales, Christian Carøe, Marcela Sandoval-Velasco, Cristina Gamba, Ross Barnett, Jose Alfredo Samaniego, Jazmin Ramos Madrigal, Ludovic Orlando, Thomas P. Gilbert

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained
    Original languageEnglish
    JournalBiotechniques
    Volume59
    Issue number6
    Pages (from-to)368-371
    Number of pages4
    ISSN0736-6205
    DOIs
    Publication statusPublished - 2015

    Fingerprint

    Dive into the research topics of 'New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA'. Together they form a unique fingerprint.

    Cite this