Abstract
An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained
Original language | English |
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Journal | BioTechniques |
Volume | 59 |
Issue number | 6 |
Pages (from-to) | 368-371 |
Number of pages | 4 |
ISSN | 0736-6205 |
DOIs | |
Publication status | Published - 2015 |