New expanded bed adsorbents for the recovery of DNA

Irini Theodossiou; M. A. Olander; M. Sondergaard; Owen R. T. Thomas

doi:10.1023/A:1026708603888

New expanded bed adsorbents for the recovery of DNA

Irini Theodossiou
, M. A. Olander
, M. Sondergaard
, Owen R. T. Thomas

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A 20-40 mum pellicular high density (similar to3.7 g cm(-3)) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml(-1), c.f. similar to 50 mug ml(-1) at 10% breakthrough), due to a combination of small particle and 'fuzzy' surface architecture created through the coupling of polyethylene imine chains.

Original language	English
Journal	Biotechnology Letters
Volume	22
Issue number	24
Pages (from-to)	1929-1933
ISSN	0141-5492
DOIs	https://doi.org/10.1023/A:1026708603888
Publication status	Published - 2000

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title = "New expanded bed adsorbents for the recovery of DNA",

abstract = "A 20-40 mum pellicular high density (similar to3.7 g cm(-3)) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml(-1), c.f. similar to 50 mug ml(-1) at 10\% breakthrough), due to a combination of small particle and 'fuzzy' surface architecture created through the coupling of polyethylene imine chains. ",

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AU - Theodossiou, Irini

AU - Olander, M. A.

AU - Sondergaard, M.

AU - Thomas, Owen R. T.

PY - 2000

Y1 - 2000

N2 - A 20-40 mum pellicular high density (similar to3.7 g cm(-3)) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml(-1), c.f. similar to 50 mug ml(-1) at 10% breakthrough), due to a combination of small particle and 'fuzzy' surface architecture created through the coupling of polyethylene imine chains.

AB - A 20-40 mum pellicular high density (similar to3.7 g cm(-3)) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml(-1), c.f. similar to 50 mug ml(-1) at 10% breakthrough), due to a combination of small particle and 'fuzzy' surface architecture created through the coupling of polyethylene imine chains.

U2 - 10.1023/A:1026708603888

DO - 10.1023/A:1026708603888

M3 - Journal article

SN - 0141-5492

VL - 22

SP - 1929

EP - 1933

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 24

ER -

New expanded bed adsorbents for the recovery of DNA

Abstract

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