New expanded bed adsorbents for the recovery of DNA

Irini Theodossiou, M. A. Olander, M. Sondergaard, Owen R. T. Thomas

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A 20-40 mum pellicular high density (similar to3.7 g cm(-3)) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml(-1), c.f. similar to 50 mug ml(-1) at 10% breakthrough), due to a combination of small particle and 'fuzzy' surface architecture created through the coupling of polyethylene imine chains.
    Original languageEnglish
    JournalBiotechnology Letters
    Volume22
    Issue number24
    Pages (from-to)1929-1933
    ISSN0141-5492
    DOIs
    Publication statusPublished - 2000

    Fingerprint Dive into the research topics of 'New expanded bed adsorbents for the recovery of DNA'. Together they form a unique fingerprint.

    Cite this