New approach of long-term modification of Topas® to acquire surface hydrophilicity for chromosome spreading

Olga Mednova, Dorota Kwasny, Noemi Rozlosnik, Winnie Edith Svendsen, Kristoffer Almdal

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A modified and improved photografting procedure of Topas® surface hydrophilization is investigated in order to obtain stable modification of the polymer for long term storage. The achieved hydrophilicity and monitoring of the wettability during one month of storage are presented as well as a description of the optimal cleaning procedure and storage conditions to maintain the modified surface. Three minutes of oxygen plasma activation followed by 4 min of acrylic acid UV-photografting at 50 °C leads to the most stable hydrophilicity that was characterized by an initial water contact angle of 53.5° ± 1.2°. Storage of the modified material in cold water at 4 °C and refraining from ultrasonic cleaning limit water contact angle increase to 5° over 30 days. In comparison with pristine hydrophobic Topas, the proposed treatment improves chromosome spreading ability significantly.
    Original languageEnglish
    JournalApplied Surface Science
    Volume292
    Pages (from-to)1045-1051
    ISSN0169-4332
    DOIs
    Publication statusPublished - 2014

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