Neutrophilic iron-oxidizing bacteria: occurrence and relevance in biological drinking water treatment

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Rapid sand filtration (RSF) is an economical way to treat anoxic groundwater around the world. It consists of groundwater aeration followed by passage through a sand filter. The oxidation and removal of ferrous iron, which is commonly found in anoxic groundwaters, is often believed to be a fully physicochemical process. However, persistently low temperatures in RSF across Denmark may negatively affect the kinetics of chemical oxidation. The slower chemical oxidation of ferrous iron may increase the chances for iron bioconversion by neutrophilic iron-oxidizing bacteria (FeOB), which are found naturally in many environments. In this study, we used a combination of a cultivation-based opposing gradient enrichment technique and 16S rRNA gene targetedmolecular tools to isolate, quantify and identify FeOB froma RSF. Themicroscopic quantification of selectively enriched FeOB cells revealed that in RSF, neutrophilic iron oxidizers were present at the level of up to 7 × 105 cells g_1 sediment. The spatial abundance and diversity of FeOB inferred by denaturing gradient gel electrophoresis fingerprinting differed greatly both between andwithin individual sand filters. The results suggest a larger than assumed role of FeOB in iron removal at waterworks using RSF technologies.
Original languageEnglish
JournalWater Science and Technology: Water Supply
Volume13
Issue number5
Pages (from-to)1295-1301
ISSN1606-9749
DOIs
Publication statusPublished - 2013

Keywords

  • Diversity
  • FeOB
  • Iron-oxidizing bacteria
  • Neutrophilic
  • Rapid sand filtration

Cite this

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title = "Neutrophilic iron-oxidizing bacteria: occurrence and relevance in biological drinking water treatment",
abstract = "Rapid sand filtration (RSF) is an economical way to treat anoxic groundwater around the world. It consists of groundwater aeration followed by passage through a sand filter. The oxidation and removal of ferrous iron, which is commonly found in anoxic groundwaters, is often believed to be a fully physicochemical process. However, persistently low temperatures in RSF across Denmark may negatively affect the kinetics of chemical oxidation. The slower chemical oxidation of ferrous iron may increase the chances for iron bioconversion by neutrophilic iron-oxidizing bacteria (FeOB), which are found naturally in many environments. In this study, we used a combination of a cultivation-based opposing gradient enrichment technique and 16S rRNA gene targetedmolecular tools to isolate, quantify and identify FeOB froma RSF. Themicroscopic quantification of selectively enriched FeOB cells revealed that in RSF, neutrophilic iron oxidizers were present at the level of up to 7 × 105 cells g_1 sediment. The spatial abundance and diversity of FeOB inferred by denaturing gradient gel electrophoresis fingerprinting differed greatly both between andwithin individual sand filters. The results suggest a larger than assumed role of FeOB in iron removal at waterworks using RSF technologies.",
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author = "Arda G{\"u}lay and Sanin Musovic and Hans-J{\o}rgen Albrechtsen and Smets, {Barth F.}",
year = "2013",
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}

Neutrophilic iron-oxidizing bacteria: occurrence and relevance in biological drinking water treatment. / Gülay, Arda; Musovic, Sanin; Albrechtsen, Hans-Jørgen; Smets, Barth F.

In: Water Science and Technology: Water Supply, Vol. 13, No. 5, 2013, p. 1295-1301.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Neutrophilic iron-oxidizing bacteria: occurrence and relevance in biological drinking water treatment

AU - Gülay, Arda

AU - Musovic, Sanin

AU - Albrechtsen, Hans-Jørgen

AU - Smets, Barth F.

PY - 2013

Y1 - 2013

N2 - Rapid sand filtration (RSF) is an economical way to treat anoxic groundwater around the world. It consists of groundwater aeration followed by passage through a sand filter. The oxidation and removal of ferrous iron, which is commonly found in anoxic groundwaters, is often believed to be a fully physicochemical process. However, persistently low temperatures in RSF across Denmark may negatively affect the kinetics of chemical oxidation. The slower chemical oxidation of ferrous iron may increase the chances for iron bioconversion by neutrophilic iron-oxidizing bacteria (FeOB), which are found naturally in many environments. In this study, we used a combination of a cultivation-based opposing gradient enrichment technique and 16S rRNA gene targetedmolecular tools to isolate, quantify and identify FeOB froma RSF. Themicroscopic quantification of selectively enriched FeOB cells revealed that in RSF, neutrophilic iron oxidizers were present at the level of up to 7 × 105 cells g_1 sediment. The spatial abundance and diversity of FeOB inferred by denaturing gradient gel electrophoresis fingerprinting differed greatly both between andwithin individual sand filters. The results suggest a larger than assumed role of FeOB in iron removal at waterworks using RSF technologies.

AB - Rapid sand filtration (RSF) is an economical way to treat anoxic groundwater around the world. It consists of groundwater aeration followed by passage through a sand filter. The oxidation and removal of ferrous iron, which is commonly found in anoxic groundwaters, is often believed to be a fully physicochemical process. However, persistently low temperatures in RSF across Denmark may negatively affect the kinetics of chemical oxidation. The slower chemical oxidation of ferrous iron may increase the chances for iron bioconversion by neutrophilic iron-oxidizing bacteria (FeOB), which are found naturally in many environments. In this study, we used a combination of a cultivation-based opposing gradient enrichment technique and 16S rRNA gene targetedmolecular tools to isolate, quantify and identify FeOB froma RSF. Themicroscopic quantification of selectively enriched FeOB cells revealed that in RSF, neutrophilic iron oxidizers were present at the level of up to 7 × 105 cells g_1 sediment. The spatial abundance and diversity of FeOB inferred by denaturing gradient gel electrophoresis fingerprinting differed greatly both between andwithin individual sand filters. The results suggest a larger than assumed role of FeOB in iron removal at waterworks using RSF technologies.

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KW - FeOB

KW - Iron-oxidizing bacteria

KW - Neutrophilic

KW - Rapid sand filtration

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