Neurotoxic effect of maneb in rats as studied by neurochemical and immunohistochemical parameters

Brian Svend Nielsen, Erik Huusfeldt Larsen, Ole Ladefoged, Henrik Rye Lam

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Epidemiological investigations document that workers in agriculture, horticulture and people living near areas with frequent use of pesticides have increased risk of developing symptoms of Parkinson's disease. This study investigated the neurotoxic effect of the fungicide maneb by morphological, immunolhistochemical and neurochemical methods applying young Sprague-Dawley male rat as the model. Intraperitoneal dosing (7.5, 15 or 30 mg maneb/kg bodyweight/week for 12 weeks) demonstrated dose-related increased manganese concentration in corpus striatum. The striatal concentration of 5-hydroxytryptamine (5-HT) increased in a dose-related manner, as did the 5-HT concentrations in the rest of the brain indicating early sign of neurotoxicity. Striatal acetylcholinesterase activity was not affected. The concentrations of noradrenaline, dopamine, neurotransmitter amino acids and the levels of the proteins alpha-synuclein and synaptophysin in corpus striatum and the rest of the brain were not changed. No histological parameter was affected when studied in corpus striatum, and substantia nigra.
Original languageEnglish
JournalEnvironmental Toxicology and Pharmacology
Issue number3
Pages (from-to)268-275
Publication statusPublished - 2006


  • 5-hydroxytryptamine
  • fungicide
  • Parkinson's disease
  • neurotoxicity
  • manganese


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