I will be presenting a powerful approach for controlling light-gated ion channels and pumps that makes it possible to probe intact neural circuits by manipulating the activity of groups of genetically similar neurons. It is experimentally demonstrated how it is now possible to precisely aiming stimulating light at single neuronal processes, neurons or groups of neurons. The underlying laser technique combines our patented Generalized Phase Contrast (GPC) with socalled temporal focusing to shape two-photon excitation for this purpose. The laser excitation patterns are generated automatically from fluorescence images of neurons and shaped to cover the cell body or dendrites, or distributed groups of cells. The temporal focusing GPC two-photon excitation patterns can generate large photocurrents in Channelrhodopsin-2–expressing cultured cells and neurons and in mouse acute cortical slices. The amplitudes of the photocurrents can be precisely modulated by controlling the size and shape of the excitation volume and, thereby, be used to trigger single action potentials or trains of action potentials. The work was recently featured in Nature Methods.
|Publication status||Published - 2010|
|Event||Hamamatsu Photonics Central Research Laboratories - Hamakita, Japan|
Duration: 1 Jan 2010 → …
|Conference||Hamamatsu Photonics Central Research Laboratories|
|Period||01/01/2010 → …|