Near-IR spectroscopic monitoring of analytes during microbially catalysed Baeyer-Villiger bioconversions

Paul A. Bird, David C. A. Sharp, John Woodley

Research output: Contribution to journalJournal articleResearchpeer-review


Sensitive and robust monitoring of product and reactants in a complex bioconversion stream is essential for the development of effective process-control strategies. In this contribution we report the use of near-infrared spectroscopy (at-line and on-line) to monitor a microbially catalysed Baeyer-Villiger bioconversion of a cyclic ketone to an optically pure lactone. The cyclohexanone monooxygenase-catalysed reaction is characterised by substrate (ketone) and product (lactone) inhibition of enzyme activity at relatively low concentrations. Quantitative multivariate calibration of a near-IR spectrophotometer for ketone and lactone resulted in a standard error of prediction at-line of 0.088 and 0.110 g/L and on-line of 0.130 and 0.180 g/L, respectively. The concentrations of substrate and product could be simultaneously monitored by near-IR, which had a response time of 5.0 and 0.75 min at-line and on-line, respectively. This work has indicated that near-IR spectroscopy has the potential to permit the realisation of an improved control strategy for this conversion based on these response times.
Original languageEnglish
JournalOrganic Process Research and Development
Issue number4
Pages (from-to)569-576
Publication statusPublished - 2002
Externally publishedYes


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