Natural Killer Cells Are Activated by Lactic Acid Bacteria-Matured Dendritic Cells

Lisbeth Nielsen Fink, Hanne Risager Christensen, Hanne Frøkiær

    Research output: Contribution to conferencePosterResearchpeer-review


    Natural killer (NK) cells are cells of the non-specific immune system lysing altered self-cells. A non-cytolytic subset of NK cells may serve a regulatory role by secreting cytokines. Bacteria translocating across the gastrointestinal mucosa are presumed to gain access to NK cells, as consumption of certain lactic acid bacteria has been shown to increase in vivo NK cytotoxicity. Here, we investigated how human gut flora-derived lactobacilli affect NK cells in vitro, by measuring proliferation and IFN-gamma production of human NK cells upon bacterial stimulation. Human peripheral blood NK cells were incubated with 10 microg/ml UV-inactivated bacteria or 10 microg/ml phytohemagglutinin (PHA) for four days. Proliferation was assessed by incorporation of radioactive thymidine into NK cell DNA. The IFN-gamma concentration was measured by ELISA. Incubation of NK cells with a Lactobacillus acidophilus strain increased the proliferation of the NK cells and induced IFN- production, both to levels comparable to PHA stimulation. The proliferative response was further enhanced with autologous monocytes present, probably because cytokines, secreted by monocytes having engulfed bacteria, stimulated the NK cells. In contrast, a Lactobacillus paracasei strain caused the NK cells to proliferate only in the presence of monocytes. These results demonstrate that various strains of lactobacilli have the capacity to activate NK cells in vitro, in a monocyte dependent or independent way. Hence, the encounter of NK cells with lactic acid bacteria will affect NK cell activation. Such activation of NK cells may potentially skew an on-going or subsequent immune response towards a Th1 response.
    Original languageEnglish
    Publication date2004
    Publication statusPublished - 2004
    Event4th Meeting of the European Mucosal Immunology Group - Lyon, France
    Duration: 8 Oct 200410 Oct 2004
    Conference number: 4


    Conference4th Meeting of the European Mucosal Immunology Group
    Internet address


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