Native chinese hamster ovary cell secretion signal peptides for production of recombinant polypeptides

Mikael Rørdam Andersen (Inventor), Irina Borodina (Inventor), Duy Duc Do (Inventor), Anne Mathilde Lund (Inventor)

Research output: Patent

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Abstract

The invention provides a genetically modified Chinese Hamster Ovary (CHO) cell for production of a recombinant polypeptide, comprising a transgene encoding a CHO-derived signal peptide fused to a recombinant polypeptide, wherein said signal peptide is capable of directing co-translational transport of said polypeptide in the cell resulting in enhanced levels of polypeptide secretion. The invention further provides a method for producing a recombinant polypeptide by using the genetically modified CHO cell. The invention further provides for the use of a CHO-derived signal peptide and a transgene encoding said signal peptide to enhance production of extracellular recombinant polypeptide by a genetically modified CHO cell.
Original languageEnglish
IPCC07K14/47; C12N15/85
Patent numberEP3323826
Filing date23/05/2018
Priority date21/11/2016
Priority numberEP20160199726
Publication statusPublished - 2018

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