Abstract
The invention provides a genetically modified Chinese Hamster Ovary (CHO) cell for production of a recombinant polypeptide, comprising a transgene encoding a CHO-derived signal peptide fused to a recombinant polypeptide, wherein said signal peptide is capable of directing co-translational transport of said polypeptide in the cell resulting in enhanced levels of polypeptide secretion. The invention further provides a method for producing a recombinant polypeptide by using the genetically modified CHO cell. The invention further provides for the use of a CHO-derived signal peptide and a transgene encoding said signal peptide to enhance production of extracellular recombinant polypeptide by a genetically modified CHO cell.
| Original language | English |
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| IPC | C07K14/47; C12N15/85 |
| Patent number | EP3323826 |
| Filing date | 23/05/2018 |
| Priority date | 21/11/2016 |
| Priority number | EP20160199726 |
| Publication status | Published - 2018 |