Naphthalene treatment alters liver intermediary metabolism and levels of steroid hormones in plasma of rainbow trout (Oncorhynchus mykiss)

Adrian Tintos, Manuel Gesto, Jesus M. Miguez, Jose L. Soengas

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

To assess the effects of naphthalene on liver intermediary metabolism and plasma steroid hormones, immature female rainbow trout (Oncorhynchus mykiss), in a first experiment, were intraperitoneally injected (2 mu L g(-1)) with vegetable oil alone (control) or containing naphthalene (10 and 50 mg kg(-1)) and returned to their tanks. At 1, 3, and 6 h after injection, eight fish were sampled from each group. A second experiment was similarly designed but used fish intraperitoneally implanted (10 mu L g(-1)) with slow-release coconut oil implants alone (control) or containing naphthalene at doses of 10 and 50mg kg(-1) body weight that were sampled 1, 3, and 5 days after injection. At each sampling time, plasma hormone levels (cortisol and 17 beta-estradiol) and metabolic parameters in plasma (glucose and lactate) and liver (glucose, lactate, and glycogen levels and HK, GK, GPase, GDH, FBPase, and PK activities) were assessed. Changes described for both hormonal systems resulted in a decrease in plasma levels of cortisol and 17 beta-estradiol. Changes observed in intermediary metabolism described effects in several pathways of liver energy metabolism due to naphthalene. These changes can be summarized as increased glycogenolysis, use of exogenous glucose, and glycolysis and decreased gluconeogenesis. The increased energy production in liver suggested by these changes can be related to the increased detoxification activity known to occcur in liver after PAH exposure, and can be also related directly or indirectly to the changes observed in the levels of plasma steroids. (c) 2006 Elsevier Inc. All rights reserved.
Original languageEnglish
JournalEcotoxicology and Environmental Safety
Volume66
Issue number2
Pages (from-to)139-147
ISSN0147-6513
DOIs
Publication statusPublished - 2007
Externally publishedYes

Keywords

  • ENVIRONMENTAL
  • TOXICOLOGY
  • ANGUILLA-ANGUILLA L.
  • POLYCYCLIC AROMATIC-HYDROCARBONS
  • ERYTHROCYTIC NUCLEAR ABNORMALITIES
  • FISH PAGOTHENIA-BORCHGREVINKI
  • FLOUNDER PLATICHTHYS-FLESUS
  • WATER-SOLUBLE FRACTION
  • BETA-NAPHTHOFLAVONE
  • ENERGY-METABOLISM
  • CARBOHYDRATE-METABOLISM
  • FUNDULUS-HETEROCLITUS
  • naphthalene
  • cortisol
  • 17 beta-estradiol
  • energy metabolism
  • rainbow trout
  • Animals
  • Blood Glucose
  • Estradiol
  • Female
  • Fructose-Bisphosphatase
  • Glucokinase
  • Glucose
  • Glutamate Dehydrogenase
  • Glycogen
  • Glycogen Phosphorylase
  • Hexokinase
  • Hydrocortisone
  • Lactic Acid
  • Liver
  • Naphthalenes
  • Oncorhynchus mykiss
  • Pyruvate Kinase
  • Asp-AT - Aspartate aminotransferase (EC. 2.6.1.1.)
  • HK - Hexokinase (EC. 2.7.1.11.)
  • FBPase - Fructose 1 - 6-bisphosphatase (EC. 3.1.3.11.)
  • GDH - Glutamate dehydrogenase (EC. 1.4.1.2.)
  • GK - Glucokinase (EC. 2.7.1.2.)
  • GPase - Glycogen phosphorylase (EC. 2.4.1.1.)
  • LDH - Lactate dehydrogenase (EC. 1.1.1.27.)
  • PK - Pyruvate kinase (EC. 2.7.1.40.)
  • Environmental Chemistry
  • Environmental Science (all)
  • 17β-Estradiol
  • Cortisol
  • Energy metabolism
  • Naphthalene
  • Rainbow trout
  • coconut oil
  • estradiol
  • glucose
  • glutamate dehydrogenase
  • glycogen
  • hydrocortisone
  • hydrogen potassium adenosine triphosphatase
  • lactic acid
  • steroid hormone
  • hormone
  • metabolism
  • plasma
  • salmonid
  • steroid
  • animal experiment
  • animal model
  • article
  • body weight
  • controlled study
  • energy yield
  • female
  • gluconeogenesis
  • glycogenolysis
  • glycolysis
  • lactate blood level
  • liver metabolism
  • metabolic disorder
  • nonhuman
  • safety
  • steroid blood level
  • intermediary metabolism
  • Pisces Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Osteichthyes [85206] Oncorhynchus mykiss species rainbow trout common immature female
  • 17-beta-estradiol 50-28-2
  • cortisol 50-23-7
  • FBPase
  • GDH
  • glucose 58367-01-4
  • glycogen 9005-79-2
  • GPase
  • lactate 113-21-3
  • naphthalene 91-20-3
  • 10060, Biochemistry studies - General
  • 10067, Biochemistry studies - Sterols and steroids
  • 10068, Biochemistry studies - Carbohydrates
  • 13002, Metabolism - General metabolism and metabolic pathways
  • 14004, Digestive system - Physiology and biochemistry
  • 15002, Blood - Blood and lymph studies
  • 15004, Blood - Blood cell studies
  • 17002, Endocrine - General
  • Chemical Coordination and Homeostasis
  • Ingestion and Assimilation
  • liver digestive system
  • plasma blood and lymphatics
  • Biochemistry and Molecular Biophysics
  • Digestive System
  • Endocrine System
  • Metabolism

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