Nanoscale Plasmonic V-Groove Waveguides for the Interrogation of Single Fluorescent Bacterial Cells

Oren Lotan, Jonathan Bar-David, Cameron Smith, Sharon Yagur-Kroll, Shimshon Belkin, Anders Kristensen, Uriel Levy

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We experimentally demonstrate the interrogation of an individual Escherichia coli cell using a nanoscale plasmonic V-groove waveguide. Several different configurations were studied. The first involved the excitation of the cell in a liquid environment because it flows on top of the waveguide nanocoupler, while the obtained fluorescence is coupled into the waveguide and collected at the other nanocoupler. The other two configurations involved the positioning of the bacterium within the nanoscale waveguide and its excitation in a dry environment either directly from the top or through waveguide modes. This is achieved by taking advantage of the waveguide properties not only for light guiding but also as a mechanical tool for trapping the bacteria within the V-grooves. The obtained results are supported by a set of numerical simulations, shedding more light on the mechanism of excitation. This demonstration paves the way for the construction of an efficient bioplasmonic chip for diverse cell-based sensing applications.
    Original languageEnglish
    JournalNano letters
    Volume17
    Pages (from-to)5481-5488
    ISSN1530-6984
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Aluminum plasmonics
    • Bio-plasmonic chips
    • Fluorescent bacterial cells
    • Bacterial biosensors
    • Nanoscale plasmonic V-groove waveguides
    • Single-bacterium excitation

    Cite this

    Lotan, Oren ; Bar-David, Jonathan ; Smith, Cameron ; Yagur-Kroll, Sharon ; Belkin, Shimshon ; Kristensen, Anders ; Levy, Uriel. / Nanoscale Plasmonic V-Groove Waveguides for the Interrogation of Single Fluorescent Bacterial Cells. In: Nano letters. 2017 ; Vol. 17. pp. 5481-5488.
    @article{0bd6d517f9a249d9a489a1895d42f1f2,
    title = "Nanoscale Plasmonic V-Groove Waveguides for the Interrogation of Single Fluorescent Bacterial Cells",
    abstract = "We experimentally demonstrate the interrogation of an individual Escherichia coli cell using a nanoscale plasmonic V-groove waveguide. Several different configurations were studied. The first involved the excitation of the cell in a liquid environment because it flows on top of the waveguide nanocoupler, while the obtained fluorescence is coupled into the waveguide and collected at the other nanocoupler. The other two configurations involved the positioning of the bacterium within the nanoscale waveguide and its excitation in a dry environment either directly from the top or through waveguide modes. This is achieved by taking advantage of the waveguide properties not only for light guiding but also as a mechanical tool for trapping the bacteria within the V-grooves. The obtained results are supported by a set of numerical simulations, shedding more light on the mechanism of excitation. This demonstration paves the way for the construction of an efficient bioplasmonic chip for diverse cell-based sensing applications.",
    keywords = "Aluminum plasmonics, Bio-plasmonic chips, Fluorescent bacterial cells, Bacterial biosensors, Nanoscale plasmonic V-groove waveguides, Single-bacterium excitation",
    author = "Oren Lotan and Jonathan Bar-David and Cameron Smith and Sharon Yagur-Kroll and Shimshon Belkin and Anders Kristensen and Uriel Levy",
    year = "2017",
    doi = "10.1021/acs.nanolett.7b02132",
    language = "English",
    volume = "17",
    pages = "5481--5488",
    journal = "Nano Letters",
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    Nanoscale Plasmonic V-Groove Waveguides for the Interrogation of Single Fluorescent Bacterial Cells. / Lotan, Oren; Bar-David, Jonathan; Smith, Cameron; Yagur-Kroll, Sharon; Belkin, Shimshon; Kristensen, Anders; Levy, Uriel.

    In: Nano letters, Vol. 17, 2017, p. 5481-5488.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Nanoscale Plasmonic V-Groove Waveguides for the Interrogation of Single Fluorescent Bacterial Cells

    AU - Lotan, Oren

    AU - Bar-David, Jonathan

    AU - Smith, Cameron

    AU - Yagur-Kroll, Sharon

    AU - Belkin, Shimshon

    AU - Kristensen, Anders

    AU - Levy, Uriel

    PY - 2017

    Y1 - 2017

    N2 - We experimentally demonstrate the interrogation of an individual Escherichia coli cell using a nanoscale plasmonic V-groove waveguide. Several different configurations were studied. The first involved the excitation of the cell in a liquid environment because it flows on top of the waveguide nanocoupler, while the obtained fluorescence is coupled into the waveguide and collected at the other nanocoupler. The other two configurations involved the positioning of the bacterium within the nanoscale waveguide and its excitation in a dry environment either directly from the top or through waveguide modes. This is achieved by taking advantage of the waveguide properties not only for light guiding but also as a mechanical tool for trapping the bacteria within the V-grooves. The obtained results are supported by a set of numerical simulations, shedding more light on the mechanism of excitation. This demonstration paves the way for the construction of an efficient bioplasmonic chip for diverse cell-based sensing applications.

    AB - We experimentally demonstrate the interrogation of an individual Escherichia coli cell using a nanoscale plasmonic V-groove waveguide. Several different configurations were studied. The first involved the excitation of the cell in a liquid environment because it flows on top of the waveguide nanocoupler, while the obtained fluorescence is coupled into the waveguide and collected at the other nanocoupler. The other two configurations involved the positioning of the bacterium within the nanoscale waveguide and its excitation in a dry environment either directly from the top or through waveguide modes. This is achieved by taking advantage of the waveguide properties not only for light guiding but also as a mechanical tool for trapping the bacteria within the V-grooves. The obtained results are supported by a set of numerical simulations, shedding more light on the mechanism of excitation. This demonstration paves the way for the construction of an efficient bioplasmonic chip for diverse cell-based sensing applications.

    KW - Aluminum plasmonics

    KW - Bio-plasmonic chips

    KW - Fluorescent bacterial cells

    KW - Bacterial biosensors

    KW - Nanoscale plasmonic V-groove waveguides

    KW - Single-bacterium excitation

    U2 - 10.1021/acs.nanolett.7b02132

    DO - 10.1021/acs.nanolett.7b02132

    M3 - Journal article

    VL - 17

    SP - 5481

    EP - 5488

    JO - Nano Letters

    JF - Nano Letters

    SN - 1530-6984

    ER -