Nanopillar-Assisted SERS Chromatography

Onur Durucan*, Kaiyu Wu, Marlitt Viehrig, Tomas Rindzevicius, Anja Boisen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


Practical implementation of SERS sensing is hindered by complexity of real-life samples, which often requires long and costly pretreatment and purification. Here, we present a novel nanopillar-assisted SERS chromatography (NPC-SERS) method for simultaneous quantitation of target molecules and analysis of complex, multicomponent fluids, e.g. human urine spiked with a model drug paracetamol (PAR). Gold-coated silicon nanopillar (AuNP) SERS substrates and a centrifugal microfluidic platform are tactfully combined, which allows (i) a precise and fully automated sample manipulation, and (ii) spatial separation of different molecular species on the AuNP substrate. The NPC-SERS technique provides a novel approach for wetting the stationary phase (AuNP) using the 'wicking effect', and thus minimizes dilution of analytes. Separation of PAR and the main human urine components (urea, uric acid and creatinine) has been demonstrated. Quantitative detection of PAR with ultra-wide linear dynamic range (0-500 ppm) is achieved by analyzing the spreading profiles of PAR on the AuNP surface. NPC-SERS transforms SERS into a sensing technique with general applicability, facilitating rapid and quantitative detection of analytes in complex biofluids, such as saliva, blood and urine.
Original languageEnglish
JournalACS Sensors
Issue number12
Pages (from-to)2492-2498
Publication statusPublished - 2018


  • Multicomponent analysis
  • Real-life samples
  • Quantitative SERS
  • Nanopillar-assisted chromatography
  • Full automation


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