Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology

Michael Bache; Filippo Bosco; Anna Line Brøgger; Kasper Bayer Frøhling; Tommy Sonne Alstrøm; En-Te Hwu; Ching-Hsiu Chen; Jesper Eugen-Olsen; Ing-Shouh Hwang; Anja Boisen

doi:10.1088/0957-4484/24/44/444011

Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology

Michael Bache, Filippo Bosco, Anna Line Brøgger, Kasper Bayer Frøhling, Tommy Sonne Alstrøm, En-Te Hwu, Ching-Hsiu Chen, Jesper Eugen-Olsen, Ing-Shouh Hwang, Anja Boisen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

In this work the use of a high-throughput nanomechanical detection system based on a DVD-ROM optical drive and cantilever sensors is presented for the detection of urokinase plasminogen activator receptor inflammatory biomarker (uPAR). Several large scale studies have linked elevated levels of soluble uPAR (suPAR) to infectious diseases, such as HIV, and certain types of cancer. Using hundreds of cantilevers and a DVD-based platform, cantilever deflection response from antibody–antigen recognition is investigated as a function of suPAR concentration. The goal is to provide a cheap and portable detection platform which can carry valuable prognostic information. In order to optimize the cantilever response the antibody immobilization and unspecific binding are initially characterized using quartz crystal microbalance technology. Also, the choice of antibody is explored in order to generate the largest surface stress on the cantilevers, thus increasing the signal. Using optimized experimental conditions the lowest detectable suPAR concentration is currently around 5 nM. The results reveal promising research strategies for the implementation of specific biochemical assays in a portable and high-throughput microsensor-based detection platform.

Original language	English
Article number	444011
Journal	Nanotechnology
Volume	24
Issue number	44
Number of pages	7
ISSN	0957-4484
DOIs	https://doi.org/10.1088/0957-4484/24/44/444011
Publication status	Published - 2013

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@article{8305c6305b3646ef8b662121b13cd517,

title = "Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology",

abstract = "In this work the use of a high-throughput nanomechanical detection system based on a DVD-ROM optical drive and cantilever sensors is presented for the detection of urokinase plasminogen activator receptor inflammatory biomarker (uPAR). Several large scale studies have linked elevated levels of soluble uPAR (suPAR) to infectious diseases, such as HIV, and certain types of cancer. Using hundreds of cantilevers and a DVD-based platform, cantilever deflection response from antibody–antigen recognition is investigated as a function of suPAR concentration. The goal is to provide a cheap and portable detection platform which can carry valuable prognostic information. In order to optimize the cantilever response the antibody immobilization and unspecific binding are initially characterized using quartz crystal microbalance technology. Also, the choice of antibody is explored in order to generate the largest surface stress on the cantilevers, thus increasing the signal. Using optimized experimental conditions the lowest detectable suPAR concentration is currently around 5 nM. The results reveal promising research strategies for the implementation of specific biochemical assays in a portable and high-throughput microsensor-based detection platform.",

author = "Michael Bache and Filippo Bosco and Br{\o}gger, {Anna Line} and Fr{\o}hling, {Kasper Bayer} and Alstr{\o}m, {Tommy Sonne} and En-Te Hwu and Ching-Hsiu Chen and Jesper Eugen-Olsen and Ing-Shouh Hwang and Anja Boisen",

year = "2013",

doi = "10.1088/0957-4484/24/44/444011",

language = "English",

volume = "24",

journal = "Nanotechnology",

issn = "0957-4484",

publisher = "IOP Publishing",

number = "44",

}

Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology. / Bache, Michael; Bosco, Filippo; Brøgger, Anna Line; Frøhling, Kasper Bayer; Alstrøm, Tommy Sonne; Hwu, En-Te; Chen, Ching-Hsiu; Eugen-Olsen, Jesper; Hwang, Ing-Shouh; Boisen, Anja.

In: Nanotechnology, Vol. 24, No. 44, 444011, 2013.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology

AU - Bache, Michael

AU - Bosco, Filippo

AU - Brøgger, Anna Line

AU - Frøhling, Kasper Bayer

AU - Alstrøm, Tommy Sonne

AU - Hwu, En-Te

AU - Chen, Ching-Hsiu

AU - Eugen-Olsen, Jesper

AU - Hwang, Ing-Shouh

AU - Boisen, Anja

PY - 2013

Y1 - 2013

N2 - In this work the use of a high-throughput nanomechanical detection system based on a DVD-ROM optical drive and cantilever sensors is presented for the detection of urokinase plasminogen activator receptor inflammatory biomarker (uPAR). Several large scale studies have linked elevated levels of soluble uPAR (suPAR) to infectious diseases, such as HIV, and certain types of cancer. Using hundreds of cantilevers and a DVD-based platform, cantilever deflection response from antibody–antigen recognition is investigated as a function of suPAR concentration. The goal is to provide a cheap and portable detection platform which can carry valuable prognostic information. In order to optimize the cantilever response the antibody immobilization and unspecific binding are initially characterized using quartz crystal microbalance technology. Also, the choice of antibody is explored in order to generate the largest surface stress on the cantilevers, thus increasing the signal. Using optimized experimental conditions the lowest detectable suPAR concentration is currently around 5 nM. The results reveal promising research strategies for the implementation of specific biochemical assays in a portable and high-throughput microsensor-based detection platform.

AB - In this work the use of a high-throughput nanomechanical detection system based on a DVD-ROM optical drive and cantilever sensors is presented for the detection of urokinase plasminogen activator receptor inflammatory biomarker (uPAR). Several large scale studies have linked elevated levels of soluble uPAR (suPAR) to infectious diseases, such as HIV, and certain types of cancer. Using hundreds of cantilevers and a DVD-based platform, cantilever deflection response from antibody–antigen recognition is investigated as a function of suPAR concentration. The goal is to provide a cheap and portable detection platform which can carry valuable prognostic information. In order to optimize the cantilever response the antibody immobilization and unspecific binding are initially characterized using quartz crystal microbalance technology. Also, the choice of antibody is explored in order to generate the largest surface stress on the cantilevers, thus increasing the signal. Using optimized experimental conditions the lowest detectable suPAR concentration is currently around 5 nM. The results reveal promising research strategies for the implementation of specific biochemical assays in a portable and high-throughput microsensor-based detection platform.

U2 - 10.1088/0957-4484/24/44/444011

DO - 10.1088/0957-4484/24/44/444011

M3 - Journal article

C2 - 24113286

VL - 24

JO - Nanotechnology

JF - Nanotechnology

SN - 0957-4484

IS - 44

M1 - 444011

ER -

Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology

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