Abstract
| Original language | English |
|---|---|
| Article number | 444011 |
| Journal | Nanotechnology |
| Volume | 24 |
| Issue number | 44 |
| Number of pages | 7 |
| ISSN | 0957-4484 |
| DOIs | |
| Publication status | Published - 2013 |
Cite this
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Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology. / Bache, Michael; Bosco, Filippo; Brøgger, Anna Line; Frøhling, Kasper Bayer; Alstrøm, Tommy Sonne; Hwu, En-Te; Chen, Ching-Hsiu; Eugen-Olsen, Jesper; Hwang, Ing-Shouh; Boisen, Anja.
In: Nanotechnology, Vol. 24, No. 44, 444011, 2013.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology
AU - Bache, Michael
AU - Bosco, Filippo
AU - Brøgger, Anna Line
AU - Frøhling, Kasper Bayer
AU - Alstrøm, Tommy Sonne
AU - Hwu, En-Te
AU - Chen, Ching-Hsiu
AU - Eugen-Olsen, Jesper
AU - Hwang, Ing-Shouh
AU - Boisen, Anja
PY - 2013
Y1 - 2013
N2 - In this work the use of a high-throughput nanomechanical detection system based on a DVD-ROM optical drive and cantilever sensors is presented for the detection of urokinase plasminogen activator receptor inflammatory biomarker (uPAR). Several large scale studies have linked elevated levels of soluble uPAR (suPAR) to infectious diseases, such as HIV, and certain types of cancer. Using hundreds of cantilevers and a DVD-based platform, cantilever deflection response from antibody–antigen recognition is investigated as a function of suPAR concentration. The goal is to provide a cheap and portable detection platform which can carry valuable prognostic information. In order to optimize the cantilever response the antibody immobilization and unspecific binding are initially characterized using quartz crystal microbalance technology. Also, the choice of antibody is explored in order to generate the largest surface stress on the cantilevers, thus increasing the signal. Using optimized experimental conditions the lowest detectable suPAR concentration is currently around 5 nM. The results reveal promising research strategies for the implementation of specific biochemical assays in a portable and high-throughput microsensor-based detection platform.
AB - In this work the use of a high-throughput nanomechanical detection system based on a DVD-ROM optical drive and cantilever sensors is presented for the detection of urokinase plasminogen activator receptor inflammatory biomarker (uPAR). Several large scale studies have linked elevated levels of soluble uPAR (suPAR) to infectious diseases, such as HIV, and certain types of cancer. Using hundreds of cantilevers and a DVD-based platform, cantilever deflection response from antibody–antigen recognition is investigated as a function of suPAR concentration. The goal is to provide a cheap and portable detection platform which can carry valuable prognostic information. In order to optimize the cantilever response the antibody immobilization and unspecific binding are initially characterized using quartz crystal microbalance technology. Also, the choice of antibody is explored in order to generate the largest surface stress on the cantilevers, thus increasing the signal. Using optimized experimental conditions the lowest detectable suPAR concentration is currently around 5 nM. The results reveal promising research strategies for the implementation of specific biochemical assays in a portable and high-throughput microsensor-based detection platform.
U2 - 10.1088/0957-4484/24/44/444011
DO - 10.1088/0957-4484/24/44/444011
M3 - Journal article
VL - 24
JO - Nanotechnology
JF - Nanotechnology
SN - 0957-4484
IS - 44
M1 - 444011
ER -