Nanoimprinted polymer chips for light induced local heating of liquids in micro- and nanochannels

    Research output: Contribution to journalConference articleResearch

    Abstract

    A nanoimprinted polymer chip with a thin near-infrared absorber layer that enables light-induced local heating (LILH) of liquids inside micro- and nanochannels is presented. An infrared laser spot and corresponding hot-spot could be scanned across the device. Large temperature gradients yield thermophoretic forces, which are used to manipulate and stretch individual DNA molecules confined in nanochannels. The absorber layer consists of a commercially available phthalocyanine dye (Fujifilm), with a narrow absorption peak at approximately 775 nm, dissolved in SU-8 photoresist (Microchem Corp.). The 500 nm thick absorber layer is spin-coated on a transparent substrate and UV exposed. Microand nanofluidic channels are defined by nanoimprint lithography in a 1.5 μm thick layer of low molecular weight polymethyl methacrylate (PMMA, Microchem Corp.), which is spin coated on top of the absorber layer. We have used a previously developed two-level hybrid stamp for replicating two V-shaped microchannels (width=50 μm and height = 900 nm) bridged by an array of 200 nanochannels (width and height of 250 nm). The fluidic channels are finally sealed with a lid using PMMA to PMMA thermal bonding. Light from a 785 nm laser diode was focused from the backside of the chip to a spot diameter down to 5 ..m in the absorber layer, yielding a localized heating (Gaussian profile) and large temperature gradients in the liquid in the nanochannels. A laser power of 38 mW yielded a temperature of 40°C in the center of a 10 μm 1/e diameter. Flourescence microscopy was performed from the frontside.
    Original languageEnglish
    JournalProceedings of the SPIE - The International Society for Optical Engineering
    Volume7764
    Pages (from-to)77640I
    ISSN0277-786X
    DOIs
    Publication statusPublished - 2010
    EventNanoengineering: Fabrication, Properties, Optics, and Devices VII - San Diego, CA, United States
    Duration: 3 Aug 20104 Aug 2010

    Conference

    ConferenceNanoengineering: Fabrication, Properties, Optics, and Devices VII
    CountryUnited States
    CitySan Diego, CA
    Period03/08/201004/08/2010

    Cite this

    @inproceedings{3cb494c74ac34ec89caf735ee15ed8f1,
    title = "Nanoimprinted polymer chips for light induced local heating of liquids in micro- and nanochannels",
    abstract = "A nanoimprinted polymer chip with a thin near-infrared absorber layer that enables light-induced local heating (LILH) of liquids inside micro- and nanochannels is presented. An infrared laser spot and corresponding hot-spot could be scanned across the device. Large temperature gradients yield thermophoretic forces, which are used to manipulate and stretch individual DNA molecules confined in nanochannels. The absorber layer consists of a commercially available phthalocyanine dye (Fujifilm), with a narrow absorption peak at approximately 775 nm, dissolved in SU-8 photoresist (Microchem Corp.). The 500 nm thick absorber layer is spin-coated on a transparent substrate and UV exposed. Microand nanofluidic channels are defined by nanoimprint lithography in a 1.5 μm thick layer of low molecular weight polymethyl methacrylate (PMMA, Microchem Corp.), which is spin coated on top of the absorber layer. We have used a previously developed two-level hybrid stamp for replicating two V-shaped microchannels (width=50 μm and height = 900 nm) bridged by an array of 200 nanochannels (width and height of 250 nm). The fluidic channels are finally sealed with a lid using PMMA to PMMA thermal bonding. Light from a 785 nm laser diode was focused from the backside of the chip to a spot diameter down to 5 ..m in the absorber layer, yielding a localized heating (Gaussian profile) and large temperature gradients in the liquid in the nanochannels. A laser power of 38 mW yielded a temperature of 40°C in the center of a 10 μm 1/e diameter. Flourescence microscopy was performed from the frontside.",
    author = "Thamdrup, {Lasse H{\o}jlund} and Pedersen, {Jonas Nyvold} and Henrik Flyvbjerg and Larsen, {Niels Bent} and Anders Kristensen",
    year = "2010",
    doi = "10.1117/12.860221",
    language = "English",
    volume = "7764",
    pages = "77640I",
    journal = "Proceedings of SPIE, the International Society for Optical Engineering",
    issn = "0277-786X",
    publisher = "S P I E - International Society for Optical Engineering",

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    TY - GEN

    T1 - Nanoimprinted polymer chips for light induced local heating of liquids in micro- and nanochannels

    AU - Thamdrup, Lasse Højlund

    AU - Pedersen, Jonas Nyvold

    AU - Flyvbjerg, Henrik

    AU - Larsen, Niels Bent

    AU - Kristensen, Anders

    PY - 2010

    Y1 - 2010

    N2 - A nanoimprinted polymer chip with a thin near-infrared absorber layer that enables light-induced local heating (LILH) of liquids inside micro- and nanochannels is presented. An infrared laser spot and corresponding hot-spot could be scanned across the device. Large temperature gradients yield thermophoretic forces, which are used to manipulate and stretch individual DNA molecules confined in nanochannels. The absorber layer consists of a commercially available phthalocyanine dye (Fujifilm), with a narrow absorption peak at approximately 775 nm, dissolved in SU-8 photoresist (Microchem Corp.). The 500 nm thick absorber layer is spin-coated on a transparent substrate and UV exposed. Microand nanofluidic channels are defined by nanoimprint lithography in a 1.5 μm thick layer of low molecular weight polymethyl methacrylate (PMMA, Microchem Corp.), which is spin coated on top of the absorber layer. We have used a previously developed two-level hybrid stamp for replicating two V-shaped microchannels (width=50 μm and height = 900 nm) bridged by an array of 200 nanochannels (width and height of 250 nm). The fluidic channels are finally sealed with a lid using PMMA to PMMA thermal bonding. Light from a 785 nm laser diode was focused from the backside of the chip to a spot diameter down to 5 ..m in the absorber layer, yielding a localized heating (Gaussian profile) and large temperature gradients in the liquid in the nanochannels. A laser power of 38 mW yielded a temperature of 40°C in the center of a 10 μm 1/e diameter. Flourescence microscopy was performed from the frontside.

    AB - A nanoimprinted polymer chip with a thin near-infrared absorber layer that enables light-induced local heating (LILH) of liquids inside micro- and nanochannels is presented. An infrared laser spot and corresponding hot-spot could be scanned across the device. Large temperature gradients yield thermophoretic forces, which are used to manipulate and stretch individual DNA molecules confined in nanochannels. The absorber layer consists of a commercially available phthalocyanine dye (Fujifilm), with a narrow absorption peak at approximately 775 nm, dissolved in SU-8 photoresist (Microchem Corp.). The 500 nm thick absorber layer is spin-coated on a transparent substrate and UV exposed. Microand nanofluidic channels are defined by nanoimprint lithography in a 1.5 μm thick layer of low molecular weight polymethyl methacrylate (PMMA, Microchem Corp.), which is spin coated on top of the absorber layer. We have used a previously developed two-level hybrid stamp for replicating two V-shaped microchannels (width=50 μm and height = 900 nm) bridged by an array of 200 nanochannels (width and height of 250 nm). The fluidic channels are finally sealed with a lid using PMMA to PMMA thermal bonding. Light from a 785 nm laser diode was focused from the backside of the chip to a spot diameter down to 5 ..m in the absorber layer, yielding a localized heating (Gaussian profile) and large temperature gradients in the liquid in the nanochannels. A laser power of 38 mW yielded a temperature of 40°C in the center of a 10 μm 1/e diameter. Flourescence microscopy was performed from the frontside.

    U2 - 10.1117/12.860221

    DO - 10.1117/12.860221

    M3 - Conference article

    VL - 7764

    SP - 77640I

    JO - Proceedings of SPIE, the International Society for Optical Engineering

    JF - Proceedings of SPIE, the International Society for Optical Engineering

    SN - 0277-786X

    ER -