Nanoimprinted distributed feedback dye laser sensor for real-time imaging of small molecule diffusion

Christoph Vannahme, Martin Dufva, Anders Kristensen

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    Abstract

    Label-free imaging is a promising tool for the study of biological processes such as cell adhesion and small molecule signaling processes. In order to image in two dimensions of space current solutions require motorized stages which results in low imaging frame rates. Here, a highly sensitive distributed feedback (DFB) dye laser sensor for real-time label-free imaging without any moving parts enabling a frame rate of 12 Hz is presented. The presence of molecules on the laser surface results in a wavelength shift which is used as sensor signal. The unique DFB laser structure comprises several areas of different grating periods which result in distinct laser emission wavelengths. Imaging in two dimensions of space is enabled by focusing an image of the laser surface with a cylindrical lens onto the entrance slit of an imaging spectrometer. Imaging is demonstrated by monitoring of diffusing small sucrose molecules in water.
    Original languageEnglish
    Title of host publication2014 IEEE SENSORS
    Number of pages3
    PublisherIEEE
    Publication date2014
    Pages1384-1386
    ISBN (Print)9781479901623
    DOIs
    Publication statusPublished - 2014
    EventIEEE Sensors 2014 - Valencia, Spain
    Duration: 2 Nov 20145 Nov 2014

    Conference

    ConferenceIEEE Sensors 2014
    CountrySpain
    CityValencia
    Period02/11/201405/11/2014

    Keywords

    • General Topics for Engineers
    • DFB laser
    • diffusion
    • dye laser
    • Label-free imaging
    • laser sensor
    • small molecule detection

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