Nanofluidic Devices with Two Pores in Series for Resistive-Pulse Sensing of Single Virus Capsids

Zachary D. Harms; Klaus Bo Mogensen; Pedro André Rodrigues de Sousa Nunes; Kaimeng Zhou; Brett W. Hildenbrand; Indranil Mitra; Zhenning Tan; Adam Zlotnick; Jörg Peter Kutter; Stephen C. Jacobson

doi:10.1021/ac202358t

Nanofluidic Devices with Two Pores in Series for Resistive-Pulse Sensing of Single Virus Capsids

Zachary D. Harms, Klaus Bo Mogensen, Pedro André Rodrigues de Sousa Nunes, Kaimeng Zhou, Brett W. Hildenbrand, Indranil Mitra, Zhenning Tan, Adam Zlotnick, Jörg Peter Kutter, Stephen C. Jacobson

Indiana University-Purdue University Indianapolis

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We report fabrication and characterization of nanochannel devices with two nanopores in series for resistive-pulse sensing of hepatitis B virus (HBV) capsids. The nanochannel and two pores are patterned by electron beam lithography between two microchannels and etched by reactive ion etching. The two nanopores are 50-nm wide, 50-nm deep, and 40-nm long and are spaced 2.0-μm apart. The nanochannel that brackets the two pores is 20 wider (1 μm) to reduce the electrical resistance adjacent to the two pores and to ensure the current returns to its baseline value between resistive-pulse events. Average pulse amplitudes differ by

Original language	English
Journal	Analytical Chemistry
Volume	83
Pages (from-to)	9573-9578
ISSN	0003-2700
DOIs	https://doi.org/10.1021/ac202358t
Publication status	Published - 2011

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title = "Nanofluidic Devices with Two Pores in Series for Resistive-Pulse Sensing of Single Virus Capsids",

abstract = "We report fabrication and characterization of nanochannel devices with two nanopores in series for resistive-pulse sensing of hepatitis B virus (HBV) capsids. The nanochannel and two pores are patterned by electron beam lithography between two microchannels and etched by reactive ion etching. The two nanopores are 50-nm wide, 50-nm deep, and 40-nm long and are spaced 2.0-μm apart. The nanochannel that brackets the two pores is 20 wider (1 μm) to reduce the electrical resistance adjacent to the two pores and to ensure the current returns to its baseline value between resistive-pulse events. Average pulse amplitudes differ by ",

author = "Harms, \{Zachary D.\} and Mogensen, \{Klaus Bo\} and \{Rodrigues de Sousa Nunes\}, \{Pedro Andr{\'e}\} and Kaimeng Zhou and Hildenbrand, \{Brett W.\} and Indranil Mitra and Zhenning Tan and Adam Zlotnick and Kutter, \{J{\"o}rg Peter\} and Jacobson, \{Stephen C.\}",

year = "2011",

doi = "10.1021/ac202358t",

language = "English",

volume = "83",

pages = "9573--9578",

journal = "Analytical Chemistry",

issn = "0003-2700",

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T1 - Nanofluidic Devices with Two Pores in Series for Resistive-Pulse Sensing of Single Virus Capsids

AU - Harms, Zachary D.

AU - Mogensen, Klaus Bo

AU - Rodrigues de Sousa Nunes, Pedro André

AU - Zhou, Kaimeng

AU - Hildenbrand, Brett W.

AU - Mitra, Indranil

AU - Tan, Zhenning

AU - Zlotnick, Adam

AU - Kutter, Jörg Peter

AU - Jacobson, Stephen C.

PY - 2011

Y1 - 2011

N2 - We report fabrication and characterization of nanochannel devices with two nanopores in series for resistive-pulse sensing of hepatitis B virus (HBV) capsids. The nanochannel and two pores are patterned by electron beam lithography between two microchannels and etched by reactive ion etching. The two nanopores are 50-nm wide, 50-nm deep, and 40-nm long and are spaced 2.0-μm apart. The nanochannel that brackets the two pores is 20 wider (1 μm) to reduce the electrical resistance adjacent to the two pores and to ensure the current returns to its baseline value between resistive-pulse events. Average pulse amplitudes differ by

AB - We report fabrication and characterization of nanochannel devices with two nanopores in series for resistive-pulse sensing of hepatitis B virus (HBV) capsids. The nanochannel and two pores are patterned by electron beam lithography between two microchannels and etched by reactive ion etching. The two nanopores are 50-nm wide, 50-nm deep, and 40-nm long and are spaced 2.0-μm apart. The nanochannel that brackets the two pores is 20 wider (1 μm) to reduce the electrical resistance adjacent to the two pores and to ensure the current returns to its baseline value between resistive-pulse events. Average pulse amplitudes differ by

U2 - 10.1021/ac202358t

DO - 10.1021/ac202358t

M3 - Journal article

C2 - 22029283

SN - 0003-2700

VL - 83

SP - 9573

EP - 9578

JO - Analytical Chemistry

JF - Analytical Chemistry

ER -

Nanofluidic Devices with Two Pores in Series for Resistive-Pulse Sensing of Single Virus Capsids

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