Mycotoxins and Other Secondary Metabolites Produced in vitro by Penicillium paneum Frisvad and Penicillium roqueforti Thom Isolated from Baled Grass Silage in Ireland

Martin O'Brien, Kristian Fog Nielsen, Padraig O'Kiely, Patrick D. Forristal, Hubert Fuller, Jens Christian Frisvad

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    Secondary metabolites produced by Penicillium paneum and Penicillium roqueforti from baled grass silage were analyzed. A total of 157 isolates were investigated, comprising 78 P. paneum and 79 P. roqueforti isolates randomly selected from more than 900 colonies cultured from bales. The findings mostly agreed with the literature, although some metabolites were not consistently produced by either fungus. Roquefortine C, marcfortine A, and andrastin A were consistently produced, whereas PR toxin and patulin were not. Five silage samples were screened for fungal metabolites, with two visually moldy samples containing up to 20 mg/kg of roquefortine C, mycophenolic acid, and andrastin A along with minor quantities (0.1-5 mg/kg) of roquefortines A, B, and D, festuclavine, marcfortine A, and agroclavine. Three visually nonmoldy samples contained low amounts of mycophenolic acid and andrastin A. The ability of both molds to produce a diverse range of secondary metabolites in vitro and in silage should be a concern to livestock producers.
    Original languageEnglish
    JournalJournal of Agricultural and Food Chemistry
    Volume54
    Issue number24
    Pages (from-to)9268-9276
    ISSN0021-8561
    DOIs
    Publication statusPublished - 2006

    Keywords

    • Penicillium paneum
    • Penicillium roqueforti
    • Baled grass silage
    • Secondary metabolites
    • Patulin
    • Roquefortine C
    • Mycophenolic acid
    • PR toxin
    • Andrastin A

    Cite this

    @article{941e66345a4c4680af65e2c9145c4afc,
    title = "Mycotoxins and Other Secondary Metabolites Produced in vitro by Penicillium paneum Frisvad and Penicillium roqueforti Thom Isolated from Baled Grass Silage in Ireland",
    abstract = "Secondary metabolites produced by Penicillium paneum and Penicillium roqueforti from baled grass silage were analyzed. A total of 157 isolates were investigated, comprising 78 P. paneum and 79 P. roqueforti isolates randomly selected from more than 900 colonies cultured from bales. The findings mostly agreed with the literature, although some metabolites were not consistently produced by either fungus. Roquefortine C, marcfortine A, and andrastin A were consistently produced, whereas PR toxin and patulin were not. Five silage samples were screened for fungal metabolites, with two visually moldy samples containing up to 20 mg/kg of roquefortine C, mycophenolic acid, and andrastin A along with minor quantities (0.1-5 mg/kg) of roquefortines A, B, and D, festuclavine, marcfortine A, and agroclavine. Three visually nonmoldy samples contained low amounts of mycophenolic acid and andrastin A. The ability of both molds to produce a diverse range of secondary metabolites in vitro and in silage should be a concern to livestock producers.",
    keywords = "Penicillium paneum, Penicillium roqueforti, Baled grass silage, Secondary metabolites, Patulin, Roquefortine C, Mycophenolic acid, PR toxin, Andrastin A",
    author = "Martin O'Brien and Nielsen, {Kristian Fog} and Padraig O'Kiely and Forristal, {Patrick D.} and Hubert Fuller and Frisvad, {Jens Christian}",
    year = "2006",
    doi = "10.1021/jf0621018",
    language = "English",
    volume = "54",
    pages = "9268--9276",
    journal = "Journal of Agricultural and Food Chemistry",
    issn = "0021-8561",
    publisher = "American Chemical Society",
    number = "24",

    }

    Mycotoxins and Other Secondary Metabolites Produced in vitro by Penicillium paneum Frisvad and Penicillium roqueforti Thom Isolated from Baled Grass Silage in Ireland. / O'Brien, Martin; Nielsen, Kristian Fog; O'Kiely, Padraig; Forristal, Patrick D.; Fuller, Hubert; Frisvad, Jens Christian.

    In: Journal of Agricultural and Food Chemistry, Vol. 54, No. 24, 2006, p. 9268-9276.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Mycotoxins and Other Secondary Metabolites Produced in vitro by Penicillium paneum Frisvad and Penicillium roqueforti Thom Isolated from Baled Grass Silage in Ireland

    AU - O'Brien, Martin

    AU - Nielsen, Kristian Fog

    AU - O'Kiely, Padraig

    AU - Forristal, Patrick D.

    AU - Fuller, Hubert

    AU - Frisvad, Jens Christian

    PY - 2006

    Y1 - 2006

    N2 - Secondary metabolites produced by Penicillium paneum and Penicillium roqueforti from baled grass silage were analyzed. A total of 157 isolates were investigated, comprising 78 P. paneum and 79 P. roqueforti isolates randomly selected from more than 900 colonies cultured from bales. The findings mostly agreed with the literature, although some metabolites were not consistently produced by either fungus. Roquefortine C, marcfortine A, and andrastin A were consistently produced, whereas PR toxin and patulin were not. Five silage samples were screened for fungal metabolites, with two visually moldy samples containing up to 20 mg/kg of roquefortine C, mycophenolic acid, and andrastin A along with minor quantities (0.1-5 mg/kg) of roquefortines A, B, and D, festuclavine, marcfortine A, and agroclavine. Three visually nonmoldy samples contained low amounts of mycophenolic acid and andrastin A. The ability of both molds to produce a diverse range of secondary metabolites in vitro and in silage should be a concern to livestock producers.

    AB - Secondary metabolites produced by Penicillium paneum and Penicillium roqueforti from baled grass silage were analyzed. A total of 157 isolates were investigated, comprising 78 P. paneum and 79 P. roqueforti isolates randomly selected from more than 900 colonies cultured from bales. The findings mostly agreed with the literature, although some metabolites were not consistently produced by either fungus. Roquefortine C, marcfortine A, and andrastin A were consistently produced, whereas PR toxin and patulin were not. Five silage samples were screened for fungal metabolites, with two visually moldy samples containing up to 20 mg/kg of roquefortine C, mycophenolic acid, and andrastin A along with minor quantities (0.1-5 mg/kg) of roquefortines A, B, and D, festuclavine, marcfortine A, and agroclavine. Three visually nonmoldy samples contained low amounts of mycophenolic acid and andrastin A. The ability of both molds to produce a diverse range of secondary metabolites in vitro and in silage should be a concern to livestock producers.

    KW - Penicillium paneum

    KW - Penicillium roqueforti

    KW - Baled grass silage

    KW - Secondary metabolites

    KW - Patulin

    KW - Roquefortine C

    KW - Mycophenolic acid

    KW - PR toxin

    KW - Andrastin A

    U2 - 10.1021/jf0621018

    DO - 10.1021/jf0621018

    M3 - Journal article

    VL - 54

    SP - 9268

    EP - 9276

    JO - Journal of Agricultural and Food Chemistry

    JF - Journal of Agricultural and Food Chemistry

    SN - 0021-8561

    IS - 24

    ER -