Abstract
We describe a quantitative detection method for mutated microRNA in human plasma samples. Specific oligonucleotides designed from a Peyrard-Bishop model allowed accurate prediction of target:probe recognition affinity and specificity. Our amplification-free tandem bead-based hybridization assay had limit of detection of 2.2 pM. Thereby, the assay allowed identification of single-nucleotide polymorphism mismatch profiles in clinically relevant microRNA-128-2-3p, showing terminal mutations that correlate positively with inflammatory colitis and colorectal cancer.
Original language | English |
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Article number | e0289556 |
Journal | PLOS ONE |
Volume | 18 |
Number of pages | 16 |
ISSN | 1932-6203 |
DOIs | |
Publication status | Published - 2023 |