Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay

Sofie Slott, Cecilie Schiøth Krüger-Jensen, Izabela Ferreira da Silva, Nadia Bom Pedersen, Kira Astakhova*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

54 Downloads (Pure)

Abstract

We describe a quantitative detection method for mutated microRNA in human plasma samples. Specific oligonucleotides designed from a Peyrard-Bishop model allowed accurate prediction of target:probe recognition affinity and specificity. Our amplification-free tandem bead-based hybridization assay had limit of detection of 2.2 pM. Thereby, the assay allowed identification of single-nucleotide polymorphism mismatch profiles in clinically relevant microRNA-128-2-3p, showing terminal mutations that correlate positively with inflammatory colitis and colorectal cancer.

Original languageEnglish
Article numbere0289556
JournalPLOS ONE
Volume18
Number of pages16
ISSN1932-6203
DOIs
Publication statusPublished - 2023

Fingerprint

Dive into the research topics of 'Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay'. Together they form a unique fingerprint.

Cite this