Mutant rnase e for enhancing recombinant protein expression

Morten Helge Hauberg Nørholm (Inventor), Sophia Andrea Helga Heyde (Inventor)

Research output: Patent

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Abstract

The invention provides a microbial host cell for enhanced recombinant expression of a target protein, said host cell comprising a mutant RNase E enzyme to be coexpressed with a target gene of interest. The invention further provides a method of enhancing recombinant protein expression using said microbial host cell. The method is particularly useful for the expression of proteins that are otherwise difficult to express in traditional expression systems, such as proteins which are toxic to the host cell. The invention further provides an auxiliary plasmid comprising a rne* gene encoding a mutant RNase E enzyme and a LysS gene encoding T7 lysozyme.

Original languageEnglish
IPCC12P 21/ 02 A I
Patent numberWO2022253962
Filing date02/06/2021
Country/TerritoryInternational Bureau of the World Intellectual Property Organization (WIPO)
Priority date02/06/2021
Priority numberEP20210177466
Publication statusPublished - 8 Dec 2022

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