TY - RPRT
T1 - Mutagenesis of the model grass Brachypodium distachyon with sodium azide
AU - Engvild, K.C.
PY - 2005
Y1 - 2005
N2 - The model plant Brachypodium distachyon (L) Beauv. can be difficult to mutate by chemical mutagens. Three diploid (2n=10) accessions of this model of cereals and temperate grasses varied widely in their sensitivity to mutagenesis by acidified sodium azide and ethyl methanesulfonate (EMS). Azide was an effective mutagen at high doses. EMS was ineffective. One accession, BDR018, showed essentially no chlorophyll mutants in the M2 generation; another, BDR001, gave only albinos, while BDR037 showed the common types of chlorophyll mutants of whites, yellows, light greens and striped in the M2 generation. The highest rate was chlorophyll mutants in 45 per cent of the progenies of the M1 plants, corresponding to 5 percent of the M2 plants.
AB - The model plant Brachypodium distachyon (L) Beauv. can be difficult to mutate by chemical mutagens. Three diploid (2n=10) accessions of this model of cereals and temperate grasses varied widely in their sensitivity to mutagenesis by acidified sodium azide and ethyl methanesulfonate (EMS). Azide was an effective mutagen at high doses. EMS was ineffective. One accession, BDR018, showed essentially no chlorophyll mutants in the M2 generation; another, BDR001, gave only albinos, while BDR037 showed the common types of chlorophyll mutants of whites, yellows, light greens and striped in the M2 generation. The highest rate was chlorophyll mutants in 45 per cent of the progenies of the M1 plants, corresponding to 5 percent of the M2 plants.
KW - 9-B risiko
KW - Risø-R-1510
KW - Risø-R-1510(EN)
M3 - Report
SN - 87-550-3422-5
T3 - Denmark. Forskningscenter Risoe. Risoe-R
BT - Mutagenesis of the model grass Brachypodium distachyon with sodium azide
PB - Risø National Laboratory
CY - Roskilde
ER -