The model plant Brachypodium distachyon (L) Beauv. can be difficult to mutate by chemical mutagens. Three diploid (2n=10) accessions of this model of cereals and temperate grasses varied widely in their sensitivity to mutagenesis by acidified sodium azide and ethyl methanesulfonate (EMS). Azide was an effective mutagen at high doses. EMS was ineffective. One accession, BDR018, showed essentially no chlorophyll mutants in the M2 generation; another, BDR001, gave only albinos, while BDR037 showed the common types of chlorophyll mutants of whites, yellows, light greens and striped in the M2 generation. The highest rate was chlorophyll mutants in 45 per cent of the progenies of the M1 plants, corresponding to 5 percent of the M2 plants.
|Place of Publication||Roskilde|
|Publisher||Risø National Laboratory|
|Number of pages||9|
|Publication status||Published - 2005|
|Series||Denmark. Forskningscenter Risoe. Risoe-R|