Mutagenesis of the model grass Brachypodium distachyon with sodium azide

K.C. Engvild

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    Abstract

    The model plant Brachypodium distachyon (L) Beauv. can be difficult to mutate by chemical mutagens. Three diploid (2n=10) accessions of this model of cereals and temperate grasses varied widely in their sensitivity to mutagenesis by acidified sodium azide and ethyl methanesulfonate (EMS). Azide was an effective mutagen at high doses. EMS was ineffective. One accession, BDR018, showed essentially no chlorophyll mutants in the M2 generation; another, BDR001, gave only albinos, while BDR037 showed the common types of chlorophyll mutants of whites, yellows, light greens and striped in the M2 generation. The highest rate was chlorophyll mutants in 45 per cent of the progenies of the M1 plants, corresponding to 5 percent of the M2 plants.
    Original languageEnglish
    Place of PublicationRoskilde
    PublisherRisø National Laboratory
    Number of pages9
    ISBN (Print)87-550-3422-5
    Publication statusPublished - 2005
    SeriesDenmark. Forskningscenter Risoe. Risoe-R
    Number1510(EN)
    ISSN0106-2840

    Keywords

    • Risø-R-1510
    • Risø-R-1510(EN)

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