Muscle fibre morphology and microarchitecture in cerebral palsy patients obtained by 3D synchrotron X-ray computed tomography

Leise Borg*, Jon Sporring, Erik B. Dam, Vedrana A. Dahl, Tim B. Dyrby, Robert Feidenhans'l, Anders B. Dahl, Jessica Pingel

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Background: Synchrotron X-ray computed tomography (SXCT) allows for three-dimensional imaging of objects at a very high resolution and in large field-of-view. Purpose: The aim of this study was to use SXCT imaging for morphological analysis of muscle tissue, in order to investigate whether the analysis reveals complementary information to two-dimensional microscopy. Methods: Three-dimensional SXCT images of muscle biopsies were taken from participants with cerebral palsy and from healthy controls. We designed morphological measures from the two-dimensional slices and three-dimensional volumes of the images and measured the muscle fibre organization, which we term orientation consistency. Results: The muscle fibre cross-sectional areas were significantly larger in healthy participants than in participants with cerebral palsy when carrying out the analysis in three dimensions. However, a similar analysis carried out in two dimensions revealed no patient group difference. The present study also showed that three-dimensional orientation consistency was significantly larger for healthy participants than for participants with cerebral palsy. Conclusion: Individuals with CP have smaller muscle fibres than healthy control individuals. We argue that morphometric measures of muscle fibres in two dimensions are generally trustworthy only if the fibres extend perpendicularly to the slice plane, and otherwise three-dimensional aspects should be considered. In addition, the muscle tissue of individuals with CP showed a decreased level of orientation consistency when compared to healthy control tissue. We suggest that the observed disorganization of the tissue may be induced by atrophy caused by physical inactivity and insufficient neural activation.

Original languageEnglish
JournalComputers in Biology and Medicine
Volume107
Pages (from-to)265-269
ISSN0010-4825
DOIs
Publication statusPublished - 1 Apr 2019

Keywords

  • Cerebral palsy
  • Morphology
  • Muscle
  • Orientation consistency
  • Synchrotron computed tomography
  • Three-dimensional images

Cite this

@article{bd3abe2d79954a0a9e77c0ae7fb6f584,
title = "Muscle fibre morphology and microarchitecture in cerebral palsy patients obtained by 3D synchrotron X-ray computed tomography",
abstract = "Background: Synchrotron X-ray computed tomography (SXCT) allows for three-dimensional imaging of objects at a very high resolution and in large field-of-view. Purpose: The aim of this study was to use SXCT imaging for morphological analysis of muscle tissue, in order to investigate whether the analysis reveals complementary information to two-dimensional microscopy. Methods: Three-dimensional SXCT images of muscle biopsies were taken from participants with cerebral palsy and from healthy controls. We designed morphological measures from the two-dimensional slices and three-dimensional volumes of the images and measured the muscle fibre organization, which we term orientation consistency. Results: The muscle fibre cross-sectional areas were significantly larger in healthy participants than in participants with cerebral palsy when carrying out the analysis in three dimensions. However, a similar analysis carried out in two dimensions revealed no patient group difference. The present study also showed that three-dimensional orientation consistency was significantly larger for healthy participants than for participants with cerebral palsy. Conclusion: Individuals with CP have smaller muscle fibres than healthy control individuals. We argue that morphometric measures of muscle fibres in two dimensions are generally trustworthy only if the fibres extend perpendicularly to the slice plane, and otherwise three-dimensional aspects should be considered. In addition, the muscle tissue of individuals with CP showed a decreased level of orientation consistency when compared to healthy control tissue. We suggest that the observed disorganization of the tissue may be induced by atrophy caused by physical inactivity and insufficient neural activation.",
keywords = "Cerebral palsy, Morphology, Muscle, Orientation consistency, Synchrotron computed tomography, Three-dimensional images",
author = "Leise Borg and Jon Sporring and Dam, {Erik B.} and Dahl, {Vedrana A.} and Dyrby, {Tim B.} and Robert Feidenhans'l and Dahl, {Anders B.} and Jessica Pingel",
year = "2019",
month = "4",
day = "1",
doi = "10.1016/j.compbiomed.2019.02.008",
language = "English",
volume = "107",
pages = "265--269",
journal = "Computers in Biology and Medicine",
issn = "0010-4825",
publisher = "Pergamon Press",

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Muscle fibre morphology and microarchitecture in cerebral palsy patients obtained by 3D synchrotron X-ray computed tomography. / Borg, Leise; Sporring, Jon; Dam, Erik B.; Dahl, Vedrana A.; Dyrby, Tim B.; Feidenhans'l, Robert; Dahl, Anders B.; Pingel, Jessica.

In: Computers in Biology and Medicine, Vol. 107, 01.04.2019, p. 265-269.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Muscle fibre morphology and microarchitecture in cerebral palsy patients obtained by 3D synchrotron X-ray computed tomography

AU - Borg, Leise

AU - Sporring, Jon

AU - Dam, Erik B.

AU - Dahl, Vedrana A.

AU - Dyrby, Tim B.

AU - Feidenhans'l, Robert

AU - Dahl, Anders B.

AU - Pingel, Jessica

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Background: Synchrotron X-ray computed tomography (SXCT) allows for three-dimensional imaging of objects at a very high resolution and in large field-of-view. Purpose: The aim of this study was to use SXCT imaging for morphological analysis of muscle tissue, in order to investigate whether the analysis reveals complementary information to two-dimensional microscopy. Methods: Three-dimensional SXCT images of muscle biopsies were taken from participants with cerebral palsy and from healthy controls. We designed morphological measures from the two-dimensional slices and three-dimensional volumes of the images and measured the muscle fibre organization, which we term orientation consistency. Results: The muscle fibre cross-sectional areas were significantly larger in healthy participants than in participants with cerebral palsy when carrying out the analysis in three dimensions. However, a similar analysis carried out in two dimensions revealed no patient group difference. The present study also showed that three-dimensional orientation consistency was significantly larger for healthy participants than for participants with cerebral palsy. Conclusion: Individuals with CP have smaller muscle fibres than healthy control individuals. We argue that morphometric measures of muscle fibres in two dimensions are generally trustworthy only if the fibres extend perpendicularly to the slice plane, and otherwise three-dimensional aspects should be considered. In addition, the muscle tissue of individuals with CP showed a decreased level of orientation consistency when compared to healthy control tissue. We suggest that the observed disorganization of the tissue may be induced by atrophy caused by physical inactivity and insufficient neural activation.

AB - Background: Synchrotron X-ray computed tomography (SXCT) allows for three-dimensional imaging of objects at a very high resolution and in large field-of-view. Purpose: The aim of this study was to use SXCT imaging for morphological analysis of muscle tissue, in order to investigate whether the analysis reveals complementary information to two-dimensional microscopy. Methods: Three-dimensional SXCT images of muscle biopsies were taken from participants with cerebral palsy and from healthy controls. We designed morphological measures from the two-dimensional slices and three-dimensional volumes of the images and measured the muscle fibre organization, which we term orientation consistency. Results: The muscle fibre cross-sectional areas were significantly larger in healthy participants than in participants with cerebral palsy when carrying out the analysis in three dimensions. However, a similar analysis carried out in two dimensions revealed no patient group difference. The present study also showed that three-dimensional orientation consistency was significantly larger for healthy participants than for participants with cerebral palsy. Conclusion: Individuals with CP have smaller muscle fibres than healthy control individuals. We argue that morphometric measures of muscle fibres in two dimensions are generally trustworthy only if the fibres extend perpendicularly to the slice plane, and otherwise three-dimensional aspects should be considered. In addition, the muscle tissue of individuals with CP showed a decreased level of orientation consistency when compared to healthy control tissue. We suggest that the observed disorganization of the tissue may be induced by atrophy caused by physical inactivity and insufficient neural activation.

KW - Cerebral palsy

KW - Morphology

KW - Muscle

KW - Orientation consistency

KW - Synchrotron computed tomography

KW - Three-dimensional images

U2 - 10.1016/j.compbiomed.2019.02.008

DO - 10.1016/j.compbiomed.2019.02.008

M3 - Journal article

VL - 107

SP - 265

EP - 269

JO - Computers in Biology and Medicine

JF - Computers in Biology and Medicine

SN - 0010-4825

ER -