TY - JOUR
T1 - Murine norovirus: propagation, quantification, and genetic manipulation
AU - Hwang, Seungmin
AU - Alhatlani, Bader
AU - Arias, Armando
AU - Caddy, Sarah L
AU - Christodoulou, Constantina
AU - Cunha, Juliana Bragazza
AU - Emmott, Ed
AU - Gonzalez-Hernandez, Marta
AU - Kolawole, Abimbola
AU - Lu, Jia
AU - Rippinger, Christine
AU - Sorgeloos, Frédéric
AU - Thorne, Lucy
AU - Vashist, Surender
AU - Goodfellow, Ian
AU - Wobus, Christiane E
PY - 2014
Y1 - 2014
N2 - Murine norovirus (MNV) is a positive-sense, plus-stranded RNA virus in the Caliciviridae family. It is the most common pathogen in biomedical research colonies. MNV is also related to the human noroviruses, which cause the majority of nonbacterial gastroenteritis worldwide. Like the human noroviruses, MNV is an enteric virus that replicates in the intestine and is transmitted by the fecal-oral route. MNV replicates in murine macrophages and dendritic cells in cells in culture and in the murine host. This virus is often used to study mechanisms in norovirus biology, because human noroviruses are refractory to growth in cell culture. MNV combines the availability of a cell culture and reverse genetics system with the ability to study infection in the native host. Herein, we describe a panel of techniques that are commonly used to study MNV biology. Curr. Protoc. Microbiol 33:15K.2.1-15K.2.61. © 2014 by John Wiley & Sons, Inc.
AB - Murine norovirus (MNV) is a positive-sense, plus-stranded RNA virus in the Caliciviridae family. It is the most common pathogen in biomedical research colonies. MNV is also related to the human noroviruses, which cause the majority of nonbacterial gastroenteritis worldwide. Like the human noroviruses, MNV is an enteric virus that replicates in the intestine and is transmitted by the fecal-oral route. MNV replicates in murine macrophages and dendritic cells in cells in culture and in the murine host. This virus is often used to study mechanisms in norovirus biology, because human noroviruses are refractory to growth in cell culture. MNV combines the availability of a cell culture and reverse genetics system with the ability to study infection in the native host. Herein, we describe a panel of techniques that are commonly used to study MNV biology. Curr. Protoc. Microbiol 33:15K.2.1-15K.2.61. © 2014 by John Wiley & Sons, Inc.
KW - murine norovirus
KW - purification
KW - quantification
KW - reverse genetics
KW - transfection
U2 - 10.1002/9780471729259.mc15k02s33
DO - 10.1002/9780471729259.mc15k02s33
M3 - Journal article
C2 - 24789596
SN - 1934-8525
VL - 33
JO - Current Protocols in Microbiology
JF - Current Protocols in Microbiology
ER -