Multistaged Nanovaccines Based on Porous Silicon@Acetalated Dextran@Cancer Cell Membrane for Cancer Immunotherapy

Flavia Fontana*, Mohammad Ali Shahbazi, Dongfei Liu, Hongbo Zhang, Ermei Mäkilä, Jarno Salonen, Jouni T. Hirvonen, Hélder A. Santos

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A novel porous silicon (Psi)-based nanovaccines for cancer immunotherapy was developed by nanoprecipitation in glass capillary microfluidics. One of these systems, thermally oxidized Psi (TOPSi)@AcDEX (Acetalated dextran), was then encapsulated with vesicles derived from cancer cells, in order to combine the advantages derived from the antigenic composition of tumor lysate with the adjuvant properties of the PSi nanoparticles chosen. The other system explored, TOPSi@SpAcDEX, characterized by a positive surface charge, was surface functionalized with a model antigen (Trp2). The adjuvant nanoparticles presented high monodispersity due to the efficient mixing produced in the microfluidic device and were shown to be highly cytocompatible over two human immortalized cell lines, KG1 and BDCM. Moreover, the nanoparticles induced the expression of co-stimulatory signals both in the immortal cell lines and in peripheral blood monocytes. Finally, TOPSi@AcDEX@CCM greatly enhanced the secretion of IFN-γ in PBMC, and did not induce the secretion of IL-4, thereby orienting the polarization of the newly primed T-cells toward a Th1 cell-mediated response. Overall, the developed nanovaccines showed promising adjuvant properties and the possibility of encapsulating the nanosystems with materials derived from the patient's tumor opens new prospects in the field of personalized cancer medicine.

    Original languageEnglish
    Article number1603239
    JournalAdvanced Materials
    Volume29
    Issue number7
    Number of pages9
    ISSN0935-9648
    DOIs
    Publication statusPublished - 2017

    Keywords

    • acetalated dextran
    • cancer cell membranes
    • cancer immunotherapy
    • nanovaccines
    • porous silicon

    Fingerprint

    Dive into the research topics of 'Multistaged Nanovaccines Based on Porous Silicon@Acetalated Dextran@Cancer Cell Membrane for Cancer Immunotherapy'. Together they form a unique fingerprint.

    Cite this