Multiplex SNP genotyping using Locked Nucleic Acid and microfluidics

Yoanna Choleva; Mikkel Nørholm; Susanne Pedersen; Peter Mouritzen; Poul E. Høiby; Alex Toftgaard Nielsen; Søren Møller; Mogens H. Jakobsen; Lars Kongsbak

doi:10.1016/s1535-5535(04)00150-9

Multiplex SNP genotyping using Locked Nucleic Acid and microfluidics

Yoanna Choleva, Mikkel Nørholm, Susanne Pedersen, Peter Mouritzen, Poul E. Høiby, Alex Toftgaard Nielsen, Søren Møller, Mogens H. Jakobsen, Lars Kongsbak

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Abstract

Locked Nucleic Acid's or LNA are a new class of bicyclic DNA analogues that have a high affinity and specificity towards complementary nucleic acids. LNA containing oligonucleotides were used to develop a multiplex SNP genotyping assay based entirely on hybridization between capture probe and target. The approach incorporates a polymer microarray platform, photochemistry for immobilization of oligonucleotides onto microarrays, and a dedicated software tool to aid primer and capture probe design for highly multiplex genotyping. Furthermore, these technologies are combined in an integrated microfluidics platform for simple, highly multiplex and robust SNP genotyping.

Original language	English
Journal	SLAS TECHNOLOGY: Translating Life Sciences Innovation
Volume	6
Issue number	4
Pages (from-to)	XVI-XVII
Number of pages	6
ISSN	2472-6303
DOIs	https://doi.org/10.1016/s1535-5535(04)00150-9
Publication status	Published - 2001
Externally published	Yes

Keywords

LNA
Microarray
Microfluidics
Polymer platform
SNP genotyping

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10.1016/s1535-5535(04)00150-9

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title = "Multiplex SNP genotyping using Locked Nucleic Acid and microfluidics",

abstract = "Locked Nucleic Acid's or LNA are a new class of bicyclic DNA analogues that have a high affinity and specificity towards complementary nucleic acids. LNA containing oligonucleotides were used to develop a multiplex SNP genotyping assay based entirely on hybridization between capture probe and target. The approach incorporates a polymer microarray platform, photochemistry for immobilization of oligonucleotides onto microarrays, and a dedicated software tool to aid primer and capture probe design for highly multiplex genotyping. Furthermore, these technologies are combined in an integrated microfluidics platform for simple, highly multiplex and robust SNP genotyping.",

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doi = "10.1016/s1535-5535(04)00150-9",

language = "English",

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T1 - Multiplex SNP genotyping using Locked Nucleic Acid and microfluidics

AU - Choleva, Yoanna

AU - Nørholm, Mikkel

AU - Pedersen, Susanne

AU - Mouritzen, Peter

AU - Høiby, Poul E.

AU - Nielsen, Alex Toftgaard

AU - Møller, Søren

AU - Jakobsen, Mogens H.

AU - Kongsbak, Lars

PY - 2001

Y1 - 2001

N2 - Locked Nucleic Acid's or LNA are a new class of bicyclic DNA analogues that have a high affinity and specificity towards complementary nucleic acids. LNA containing oligonucleotides were used to develop a multiplex SNP genotyping assay based entirely on hybridization between capture probe and target. The approach incorporates a polymer microarray platform, photochemistry for immobilization of oligonucleotides onto microarrays, and a dedicated software tool to aid primer and capture probe design for highly multiplex genotyping. Furthermore, these technologies are combined in an integrated microfluidics platform for simple, highly multiplex and robust SNP genotyping.

AB - Locked Nucleic Acid's or LNA are a new class of bicyclic DNA analogues that have a high affinity and specificity towards complementary nucleic acids. LNA containing oligonucleotides were used to develop a multiplex SNP genotyping assay based entirely on hybridization between capture probe and target. The approach incorporates a polymer microarray platform, photochemistry for immobilization of oligonucleotides onto microarrays, and a dedicated software tool to aid primer and capture probe design for highly multiplex genotyping. Furthermore, these technologies are combined in an integrated microfluidics platform for simple, highly multiplex and robust SNP genotyping.

KW - LNA

KW - Microarray

KW - Microfluidics

KW - Polymer platform

KW - SNP genotyping

U2 - 10.1016/s1535-5535(04)00150-9

DO - 10.1016/s1535-5535(04)00150-9

M3 - Journal article

SN - 2472-6303

VL - 6

SP - XVI-XVII

JO - SLAS TECHNOLOGY: Translating Life Sciences Innovation

JF - SLAS TECHNOLOGY: Translating Life Sciences Innovation

IS - 4

ER -

Multiplex SNP genotyping using Locked Nucleic Acid and microfluidics

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Keywords

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