Multiplex SNP genotyping using Locked Nucleic Acid and microfluidics

Yoanna Choleva, Mikkel Nørholm, Susanne Pedersen, Peter Mouritzen, Poul E. Høiby, Alex Toftgaard Nielsen, Søren Møller, Mogens H. Jakobsen, Lars Kongsbak

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Locked Nucleic Acid's or LNA are a new class of bicyclic DNA analogues that have a high affinity and specificity towards complementary nucleic acids. LNA containing oligonucleotides were used to develop a multiplex SNP genotyping assay based entirely on hybridization between capture probe and target. The approach incorporates a polymer microarray platform, photochemistry for immobilization of oligonucleotides onto microarrays, and a dedicated software tool to aid primer and capture probe design for highly multiplex genotyping. Furthermore, these technologies are combined in an integrated microfluidics platform for simple, highly multiplex and robust SNP genotyping.
Original languageEnglish
JournalSLAS TECHNOLOGY: Translating Life Sciences Innovation
Volume6
Issue number4
Pages (from-to)XVI-XVII
Number of pages6
ISSN2472-6303
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • LNA
  • Microarray
  • Microfluidics
  • Polymer platform
  • SNP genotyping

Cite this

Choleva, Y., Nørholm, M., Pedersen, S., Mouritzen, P., Høiby, P. E., Nielsen, A. T., Møller, S., Jakobsen, M. H., & Kongsbak, L. (2001). Multiplex SNP genotyping using Locked Nucleic Acid and microfluidics. SLAS TECHNOLOGY: Translating Life Sciences Innovation, 6(4), XVI-XVII. https://doi.org/10.1016/s1535-5535(04)00150-9