Multiplex SNP genotyping using Locked Nucleic Acid and microfluidics

Yoanna Choleva, Mikkel Nørholm, Susanne Pedersen, Peter Mouritzen, Poul E. Høiby, Alex Toftgaard Nielsen, Søren Møller, Mogens H. Jakobsen, Lars Kongsbak

Research output: Contribution to journalJournal articleResearchpeer-review


Locked Nucleic Acid's or LNA are a new class of bicyclic DNA analogues that have a high affinity and specificity towards complementary nucleic acids. LNA containing oligonucleotides were used to develop a multiplex SNP genotyping assay based entirely on hybridization between capture probe and target. The approach incorporates a polymer microarray platform, photochemistry for immobilization of oligonucleotides onto microarrays, and a dedicated software tool to aid primer and capture probe design for highly multiplex genotyping. Furthermore, these technologies are combined in an integrated microfluidics platform for simple, highly multiplex and robust SNP genotyping.
Original languageEnglish
JournalSLAS TECHNOLOGY: Translating Life Sciences Innovation
Issue number4
Pages (from-to)XVI-XVII
Number of pages6
Publication statusPublished - 2001
Externally publishedYes


  • LNA
  • Microarray
  • Microfluidics
  • Polymer platform
  • SNP genotyping


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