Multiple Soluble Components of the Glycocalyx Are Increased in Patient Plasma After Ischemic Stroke

Brian DellaValle, Henrik Hasseldam, Flemming F. Johansen, Helle K. Iversen, Jørgen Rungby, Casper Hempel*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Background and Purpose- The GLX (glycocalyx) is a protein/polysaccharide meshwork at the cellular surface. Consisting largely of glycosaminoglycans and proteoglycans, the GLX can shed in response to stress. In this study, we assay 11 components of the GLX in plasma from patients with ischemic stroke from a longitudinal cohort. Methods- Plasma samples from healthy individuals (N=8), and patients with ischemic stroke day ≥3, day 7, and day 90 (N=9-14) were immunoassayed for diverse components of the GLX. Results- Median stroke severity was mild (National Institutes of Health Stroke Scale 2.0 (range, 0-6) at day ≤3). Three (keratan-chondroitin-heparan-sulfate) of 4 glycosaminoglycans and CD44 (proteoglycan) were increased at day 7 and returned to baseline at day 90. Proteoglycan syndecan (Syn)-3 increased and Syn-2 levels decreased, significantly. Conclusions- Individual GLX components are often assayed as stand-alone biomarkers for endothelial health. This study suggests a full assessment of GLX components is more indicative of the endothelial health of an individual and represents a complex GLX signature that may be valuable as a composite biomarker of disease.
Original languageEnglish
JournalStroke
Volume50
Issue number10
Pages (from-to)2948-2951
ISSN0039-2499
DOIs
Publication statusPublished - 2019

Keywords

  • Biomarkers
  • Cerebrovascular disorders
  • Glycocalyx
  • Glycosaminoglycans
  • Precision medicine
  • Proteoglycans
  • Stroke

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