Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes

I. Ventre; A.L. Goodman; I. Vallet-Gely; P. Vasseur; C. Soscia; Søren Molin; S. Bleves; A. Lazdunski; S. Lory; A. Filloux

Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes

I. Ventre, A.L. Goodman, I. Vallet-Gely, P. Vasseur, C. Soscia, Søren Molin, S. Bleves, A. Lazdunski, S. Lory, A. Filloux

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The opportunistic pathogen Pseudomonas aeruginosa is responsible for a wide range of acute and chronic infections. The transition to chronic infections is accompanied by physiological changes in the bacteria favoring formation of biofilm communities. Here we report the identification of LadS, a hybrid sensor kinase that controls the reciprocal expression of genes for type III secretion and biofilm-promoting polysaccharicles. Domain organization of LadS and the range of LadS-controlled genes suggest that it counteracts the activities of another sensor kinase, RetS. These two pathways converge by controlling the transcription of a small regulatory RNA, RsmZ. This work identifies a previously undescribed signal transduction network in which the activities of signal-receiving sensor kinases LadS, RetS, and GacS regulate expression of virulence genes associated with acute or chronic infection by transcriptional and posttranscriptional mechanisms.

Original language	English
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	103
Issue number	1
Pages (from-to)	171-176
ISSN	0027-8424
Publication status	Published - 2006

Keywords

two-component system
small RNA
biofilm
type III secretion
pel genes

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Ventre, I. ; Goodman, A.L. ; Vallet-Gely, I. ; Vasseur, P. ; Soscia, C. ; Molin, Søren ; Bleves, S. ; Lazdunski, A. ; Lory, S. ; Filloux, A. / Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 1. pp. 171-176.

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title = "Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes",

abstract = "The opportunistic pathogen Pseudomonas aeruginosa is responsible for a wide range of acute and chronic infections. The transition to chronic infections is accompanied by physiological changes in the bacteria favoring formation of biofilm communities. Here we report the identification of LadS, a hybrid sensor kinase that controls the reciprocal expression of genes for type III secretion and biofilm-promoting polysaccharicles. Domain organization of LadS and the range of LadS-controlled genes suggest that it counteracts the activities of another sensor kinase, RetS. These two pathways converge by controlling the transcription of a small regulatory RNA, RsmZ. This work identifies a previously undescribed signal transduction network in which the activities of signal-receiving sensor kinases LadS, RetS, and GacS regulate expression of virulence genes associated with acute or chronic infection by transcriptional and posttranscriptional mechanisms.",

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pages = "171--176",

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Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes. / Ventre, I.; Goodman, A.L.; Vallet-Gely, I.; Vasseur, P.; Soscia, C.; Molin, Søren; Bleves, S.; Lazdunski, A.; Lory, S.; Filloux, A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 1, 2006, p. 171-176.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes

AU - Ventre, I.

AU - Goodman, A.L.

AU - Vallet-Gely, I.

AU - Vasseur, P.

AU - Soscia, C.

AU - Molin, Søren

AU - Bleves, S.

AU - Lazdunski, A.

AU - Lory, S.

AU - Filloux, A.

PY - 2006

Y1 - 2006

N2 - The opportunistic pathogen Pseudomonas aeruginosa is responsible for a wide range of acute and chronic infections. The transition to chronic infections is accompanied by physiological changes in the bacteria favoring formation of biofilm communities. Here we report the identification of LadS, a hybrid sensor kinase that controls the reciprocal expression of genes for type III secretion and biofilm-promoting polysaccharicles. Domain organization of LadS and the range of LadS-controlled genes suggest that it counteracts the activities of another sensor kinase, RetS. These two pathways converge by controlling the transcription of a small regulatory RNA, RsmZ. This work identifies a previously undescribed signal transduction network in which the activities of signal-receiving sensor kinases LadS, RetS, and GacS regulate expression of virulence genes associated with acute or chronic infection by transcriptional and posttranscriptional mechanisms.

AB - The opportunistic pathogen Pseudomonas aeruginosa is responsible for a wide range of acute and chronic infections. The transition to chronic infections is accompanied by physiological changes in the bacteria favoring formation of biofilm communities. Here we report the identification of LadS, a hybrid sensor kinase that controls the reciprocal expression of genes for type III secretion and biofilm-promoting polysaccharicles. Domain organization of LadS and the range of LadS-controlled genes suggest that it counteracts the activities of another sensor kinase, RetS. These two pathways converge by controlling the transcription of a small regulatory RNA, RsmZ. This work identifies a previously undescribed signal transduction network in which the activities of signal-receiving sensor kinases LadS, RetS, and GacS regulate expression of virulence genes associated with acute or chronic infection by transcriptional and posttranscriptional mechanisms.

KW - two-component system

KW - small RNA

KW - biofilm

KW - type III secretion

KW - pel genes

M3 - Journal article

VL - 103

SP - 171

EP - 176

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 1

ER -