Multilocus Sequence Typing And Antibiotic Resistance Of Staphylococcus Aureus Isolated From The Brazilian Dairy Industry

Karen Kiesbye Dittmann, Luiza Chaul, Sarah Lee, Carlos Corassin, Elaine de Martinis, Virgínia Alves, Lone Gram, Virginie Oxaran

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

    Abstract

    Staphylococcus aureus is a common cause of food poisoning due to enterotoxin production. This is particularly an issue in the dairy industry, where S. aureus can contaminate the product e.g. from raw milk or the handlers. In Brazil, soft cheese is mainly produced in small dairy plants where good hygiene practices can be limited. The aim of this study was to determine if Brazilian dairy plants were contaminated by S. aureus, and if any clones were persistent. Four dairy plants were sampled during 8 months (398 samples in total). S. aureus (n=66) was found in all the dairy plants but the contamination rate varied between the processing plants. Multilocus Sequence Typing was used to type and assess potential persisting sequence types (ST). Seven known STs (ST1, ST5, ST30, ST97, ST126, ST188, ST398) were identified. Three new STs were identified and they all belong to clonal complex (CC) 1, which was the dominant CC in the investigated dairy plants. However, there were no indications of re-occurring (persistent) STs in the plants. The potential health risk of the isolates was assessed by antibiotic resistance and hemolytic activity screening. Resistance levels were low, and all of the isolates were presumptive methicillin-sensitive S. aureus. All of the isolates expressed hemolytic activity. The frequent isolation of CC1 strains in Brazilian dairy plants indicates, despite antibiotic sensitivity, a potential health risk to the human consumer.
    Original languageEnglish
    Title of host publicationThe Danish Microbiological Society Annual Congress 2015 : Programme & Abstracts
    Place of PublicationCopenhagen
    Publication date2015
    Pages57-57
    Publication statusPublished - 2015
    EventThe Danish Microbiological Society Annual Congress 2015 - Eigtved's Pakhus, Copenhagen, Denmark
    Duration: 9 Nov 20159 Nov 2015

    Conference

    ConferenceThe Danish Microbiological Society Annual Congress 2015
    LocationEigtved's Pakhus
    CountryDenmark
    CityCopenhagen
    Period09/11/201509/11/2015

    Cite this

    Dittmann, K. K., Chaul, L., Lee, S., Corassin, C., de Martinis, E., Alves, V., Gram, L., & Oxaran, V. (2015). Multilocus Sequence Typing And Antibiotic Resistance Of Staphylococcus Aureus Isolated From The Brazilian Dairy Industry. In The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts (pp. 57-57).