Multifunctionalized Gold Nanoparticles with Peptides Targeted to Gastrin-Releasing Peptide Receptor of a Tumor Cell Line

Leticia Hosta Rigau, Ivonne Olmedo, Jordi Arbiol, Luis J. Cruz, Marcelo J. Kogan, Fernando Albericio

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Functionalization of gold nanoparticles (AuNPs) with both a targeting peptide (an analogue of the peptide Bombesin) and a drug peptide ligand (an analogue of the RAF peptide) with the aim of improving selectivity in the delivery of the conjugates as well as the antitumor activity is described. Studies on the internalization mechanism of peptide-AuNP conjugates and viability of cells were carried out. An enhancement of the activity and selectivity of the peptide multifunctionalized conjugates was observed.
Original languageEnglish
JournalBioconjugate Chemistry
Volume21
Issue number6
Pages (from-to)1070-1078
Number of pages9
ISSN1043-1802
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • BIOCHEMICAL
  • BIOCHEMISTRY
  • CHEMISTRY,
  • BOMBESIN-LIKE PEPTIDES
  • IN-VIVO
  • CANCER-CELLS
  • NEUROPEPTIDE RECEPTORS
  • RB-RAF-1 INTERACTION
  • ANTAGONIST RC-3095
  • HUMAN GLIOBLASTOMA
  • HUMAN PROSTATE
  • PARTICLE-SIZE
  • LUNG-CANCER
  • Amino Acid Sequence
  • Bombesin
  • Cell Line, Tumor
  • Cell Survival
  • Fluorescent Dyes
  • Gold
  • Humans
  • Ligands
  • Metal Nanoparticles
  • Molecular Sequence Data
  • Neuroblastoma
  • Neurotransmitter Agents
  • Peptides
  • Receptors, Bombesin
  • Spectrophotometry, Ultraviolet
  • Spectroscopy, Electron Energy-Loss
  • cell viability
  • Primates Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Humans, Mammals, Primates, Vertebrates) - Hominidae [86215] HeLa cell line cell_line human cervical cancer cells SHSH-5Y cell line cell_line human neuroblastoma cells
  • bombesin analog antineoplastic-drug
  • gastrin-releasing peptide receptor GPRr expression
  • RAF peptide analog antineoplastic-drug
  • 02508, Cytology - Human
  • 12512, Pathology - Therapy
  • 22002, Pharmacology - General
  • 22005, Pharmacology - Clinical pharmacology
  • 24004, Neoplasms - Pathology, clinical aspects and systemic effects
  • 24008, Neoplasms - Therapeutic agents and therapy
  • gold nanoparticle drug delivery device
  • Pharmacology
  • Tumor Biology
  • bombesin derivative
  • gastrin releasing peptide
  • gastrin releasing peptide derivative
  • gastrin releasing peptide receptor
  • gold nanoparticle
  • antineoplastic activity
  • article
  • cancer cell culture
  • drug conjugation
  • drug receptor binding
  • HeLa cell
  • in vitro study
  • neuroblastoma
  • protein expression

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