Multidimensional analysis of protease substrates and their cellular origins in mixed secretomes from multiple cell types

Research output: Chapter in Book/Report/Conference proceedingBook chapter – Annual report year: 2017Researchpeer-review

Without internal affiliation

  • Author: Schlage, Pascal

    Swiss Federal Institute of Technology Zurich

  • Author: auf dem Keller, Ulrich

    Swiss Federal Institute of Technology Zurich

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Although extracellular proteases are confronted with substrate proteins expressed by multiple cell types in vivo, in most protease substrate discovery approaches, the test protease is exposed to a test proteome (secretome) derived only from a single cell type. This limits the potential substrate space and prohibits the formation of protein complexes constituted of components derived from multiple cellular origins. Mixing of secretomes collected from multiple cell types addresses this issue, but information on the cellular origin of a substrate protein is lost. Here, we describe a protocol and the corresponding data analysis workflow for a multidimensional substrate discovery approach termed SILAC-iTRAQ-TAILS that is based on hyperplexed terminal amine isotopic labeling of substrates (TAILS), allowing identification of substrates and concomitant assignment to cellular origins in mixed secretomes within the same experiment.

Original languageEnglish
Title of host publicationProtein Terminal Profiling
Number of pages14
PublisherHumana Press
Publication date2017
ISBN (Print)978-1-4939-6849-7
ISBN (Electronic)978-1-4939-6850-3
Publication statusPublished - 2017
Externally publishedYes
SeriesMethods in Molecular Biology
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • iTRAQ, Protease substrate, Secretome, SILAC, TAILS

ID: 140021947