Multichannel Bipotentiostat Integrated With a Microfluidic Platform for Electrochemical Real-Time Monitoring of Cell Cultures

Marco Vergani; Marco Carminati; Giorgio Ferrari; Ettore Landini; Claudia Caviglia; Arto Heiskanen; Clément Comminges; Kinga Zor; David Sabourin; Martin Dufva; Maria Dimaki; Roberto Raiteri; Ulla Wollenberger; Jenny Emnéus; Marco Sampietro

doi:10.1109/TBCAS.2012.2187783

Multichannel Bipotentiostat Integrated With a Microfluidic Platform for Electrochemical Real-Time Monitoring of Cell Cultures

Marco Vergani, Marco Carminati, Giorgio Ferrari, Ettore Landini, Claudia Caviglia, Arto Heiskanen, Clément Comminges, Kinga Zor, David Sabourin, Martin Dufva, Maria Dimaki, Roberto Raiteri, Ulla Wollenberger, Jenny Emnéus, Marco Sampietro

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Abstract

An electrochemical detection system specifically designed for multi-parameter real-time monitoring of stem cell culturing/differentiation in a microfluidic system is presented. It is composed of a very compact 24-channel electronic board, compatible with arrays of microelectrodes and coupled to a microfluidic cell culture system. A versatile data acquisition software enables performing amperometry, cyclic voltammetry and impedance spectroscopy in each of the 12 independent chambers over a 100 kHz bandwidth with current resolution down to 5 pA for 100 ms measuring time. The design of the platform, its realization and experimental characterization are reported, with emphasis on the analysis of impact of input capacitance (i.e., microelectrode size) and microfluidic pump operation on current noise. Programmable sequences of successive injections of analytes (ferricyanide and dopamine) and rinsing buffer solution as well as the impedimetric continuous tracking for seven days of the proliferation of a colony of PC12 cells are successfully demonstrated.

Original language	English
Journal	I E E E Transactions on Biomedical Circuits and Systems
Volume	6
Issue number	5
Pages (from-to)	498-507
ISSN	1932-4545
DOIs	https://doi.org/10.1109/TBCAS.2012.2187783
Publication status	Published - 2012

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Vergani, M., Carminati, M., Ferrari, G., Landini, E., Caviglia, C., Heiskanen, A., Comminges, C., Zor, K., Sabourin, D., Dufva, M., Dimaki, M., Raiteri, R., Wollenberger, U., Emnéus, J., & Sampietro, M. (2012). Multichannel Bipotentiostat Integrated With a Microfluidic Platform for Electrochemical Real-Time Monitoring of Cell Cultures. I E E E Transactions on Biomedical Circuits and Systems, 6(5), 498-507. https://doi.org/10.1109/TBCAS.2012.2187783

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title = "Multichannel Bipotentiostat Integrated With a Microfluidic Platform for Electrochemical Real-Time Monitoring of Cell Cultures",

abstract = "An electrochemical detection system specifically designed for multi-parameter real-time monitoring of stem cell culturing/differentiation in a microfluidic system is presented. It is composed of a very compact 24-channel electronic board, compatible with arrays of microelectrodes and coupled to a microfluidic cell culture system. A versatile data acquisition software enables performing amperometry, cyclic voltammetry and impedance spectroscopy in each of the 12 independent chambers over a 100 kHz bandwidth with current resolution down to 5 pA for 100 ms measuring time. The design of the platform, its realization and experimental characterization are reported, with emphasis on the analysis of impact of input capacitance (i.e., microelectrode size) and microfluidic pump operation on current noise. Programmable sequences of successive injections of analytes (ferricyanide and dopamine) and rinsing buffer solution as well as the impedimetric continuous tracking for seven days of the proliferation of a colony of PC12 cells are successfully demonstrated.",

author = "Marco Vergani and Marco Carminati and Giorgio Ferrari and Ettore Landini and Claudia Caviglia and Arto Heiskanen and Cl{\'e}ment Comminges and Kinga Zor and David Sabourin and Martin Dufva and Maria Dimaki and Roberto Raiteri and Ulla Wollenberger and Jenny Emn{\'e}us and Marco Sampietro",

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doi = "10.1109/TBCAS.2012.2187783",

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Vergani, M, Carminati, M, Ferrari, G, Landini, E, Caviglia, C, Heiskanen, A, Comminges, C, Zor, K, Sabourin, D, Dufva, M , Dimaki, M, Raiteri, R, Wollenberger, U, Emnéus, J & Sampietro, M 2012, 'Multichannel Bipotentiostat Integrated With a Microfluidic Platform for Electrochemical Real-Time Monitoring of Cell Cultures', I E E E Transactions on Biomedical Circuits and Systems, vol. 6, no. 5, pp. 498-507. https://doi.org/10.1109/TBCAS.2012.2187783

TY - JOUR

T1 - Multichannel Bipotentiostat Integrated With a Microfluidic Platform for Electrochemical Real-Time Monitoring of Cell Cultures

AU - Vergani, Marco

AU - Carminati, Marco

AU - Ferrari, Giorgio

AU - Landini, Ettore

AU - Caviglia, Claudia

AU - Heiskanen, Arto

AU - Comminges, Clément

AU - Zor, Kinga

AU - Sabourin, David

AU - Dufva, Martin

AU - Dimaki, Maria

AU - Raiteri, Roberto

AU - Wollenberger, Ulla

AU - Emnéus, Jenny

AU - Sampietro, Marco

PY - 2012

Y1 - 2012

N2 - An electrochemical detection system specifically designed for multi-parameter real-time monitoring of stem cell culturing/differentiation in a microfluidic system is presented. It is composed of a very compact 24-channel electronic board, compatible with arrays of microelectrodes and coupled to a microfluidic cell culture system. A versatile data acquisition software enables performing amperometry, cyclic voltammetry and impedance spectroscopy in each of the 12 independent chambers over a 100 kHz bandwidth with current resolution down to 5 pA for 100 ms measuring time. The design of the platform, its realization and experimental characterization are reported, with emphasis on the analysis of impact of input capacitance (i.e., microelectrode size) and microfluidic pump operation on current noise. Programmable sequences of successive injections of analytes (ferricyanide and dopamine) and rinsing buffer solution as well as the impedimetric continuous tracking for seven days of the proliferation of a colony of PC12 cells are successfully demonstrated.

AB - An electrochemical detection system specifically designed for multi-parameter real-time monitoring of stem cell culturing/differentiation in a microfluidic system is presented. It is composed of a very compact 24-channel electronic board, compatible with arrays of microelectrodes and coupled to a microfluidic cell culture system. A versatile data acquisition software enables performing amperometry, cyclic voltammetry and impedance spectroscopy in each of the 12 independent chambers over a 100 kHz bandwidth with current resolution down to 5 pA for 100 ms measuring time. The design of the platform, its realization and experimental characterization are reported, with emphasis on the analysis of impact of input capacitance (i.e., microelectrode size) and microfluidic pump operation on current noise. Programmable sequences of successive injections of analytes (ferricyanide and dopamine) and rinsing buffer solution as well as the impedimetric continuous tracking for seven days of the proliferation of a colony of PC12 cells are successfully demonstrated.

U2 - 10.1109/TBCAS.2012.2187783

DO - 10.1109/TBCAS.2012.2187783

M3 - Journal article

C2 - 23853236

SN - 1932-4545

VL - 6

SP - 498

EP - 507

JO - I E E E Transactions on Biomedical Circuits and Systems

JF - I E E E Transactions on Biomedical Circuits and Systems

IS - 5

ER -

Multichannel Bipotentiostat Integrated With a Microfluidic Platform for Electrochemical Real-Time Monitoring of Cell Cultures

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