Multi-photon microscope driven by novel green laser pump

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Multi-photon microscopy is extensively used in research due to its superior possibilities when compared to other microscopy modalities. The technique also has the possibility to advance diagnostics in clinical applications, due to its capabilities complementing existing technology in a multimodal system. However, translation is hindered due to the high cost, high training demand and large footprint of a standard setup. We show in this article that minification of the setup, while also reducing cost and complexity, is indeed possible without compromising on image quality, by using a novel diode laser replacing the commonly used conventional solid state laser as the pump for the femtosecond system driving the imaging.
Original languageEnglish
Title of host publicationProceedings of SPIE
EditorsAmmasi Periasamy, Peter T. C. So, Karsten König
Number of pages5
PublisherSPIE - International Society for Optical Engineering
Publication date2016
Article number97121M
Publication statusPublished - 2016
EventSPIE Photonics West 2016: Multiphoton Microscopy in the Biomedical Sciences XVI - Moscone Centre, San Francisco, United States
Duration: 13 Feb 201618 Feb 2016
Conference number: 9712


ConferenceSPIE Photonics West 2016
LocationMoscone Centre
Country/TerritoryUnited States
CitySan Francisco
Internet address
SeriesProceedings of SPIE, the International Society for Optical Engineering

Bibliographical note

Copyright 2016 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.


  • Multi-Photon Microscopy
  • Diode Laser
  • Contrast
  • Image Quality


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