TY - JOUR
T1 - Multi-particle three-dimensional coordinate estimation in real-time optical manipulation
AU - Dam, Jeppe Seidelin
AU - Perch-Nielsen, Ivan R.
AU - Palima, Darwin
AU - Glückstad, Jesper
PY - 2009
Y1 - 2009
N2 - We have previously shown how stereoscopic images can be obtained in our three-dimensional optical micromanipulation system [J. S. Dam et al, Opt. Express 16, 7244 (2008)]. Here, we present an extension and application of this principle to automatically gather the three-dimensional coordinates for all trapped particles with high tracking range and high reliability without requiring user calibration. Through deconvolving of the red, green, and blue colour planes to correct for bleeding between colour planes, we show that we can extend the system to also utilize green illumination, in addition to the blue and red. Applying the green colour as on-axis illumination yields redundant information for enhanced error correction, which is used to verify the gathered data, resulting in reliable coordinates as well as producing visually attractive images.
AB - We have previously shown how stereoscopic images can be obtained in our three-dimensional optical micromanipulation system [J. S. Dam et al, Opt. Express 16, 7244 (2008)]. Here, we present an extension and application of this principle to automatically gather the three-dimensional coordinates for all trapped particles with high tracking range and high reliability without requiring user calibration. Through deconvolving of the red, green, and blue colour planes to correct for bleeding between colour planes, we show that we can extend the system to also utilize green illumination, in addition to the blue and red. Applying the green colour as on-axis illumination yields redundant information for enhanced error correction, which is used to verify the gathered data, resulting in reliable coordinates as well as producing visually attractive images.
KW - three-dimensional microscopy
KW - Optical trapping and manipulation
U2 - 10.2971/jeos.2009.09045
DO - 10.2971/jeos.2009.09045
M3 - Journal article
SN - 1990-2573
VL - 4
SP - 09045
JO - Journal of the European Optical Society - Rapid Publications
JF - Journal of the European Optical Society - Rapid Publications
ER -