Multi-particle three-dimensional coordinate estimation in real-time optical manipulation

Jeppe Seidelin Dam, Ivan R. Perch-Nielsen, Darwin Palima, Jesper Glückstad

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    Abstract

    We have previously shown how stereoscopic images can be obtained in our three-dimensional optical micromanipulation system [J. S. Dam et al, Opt. Express 16, 7244 (2008)]. Here, we present an extension and application of this principle to automatically gather the three-dimensional coordinates for all trapped particles with high tracking range and high reliability without requiring user calibration. Through deconvolving of the red, green, and blue colour planes to correct for bleeding between colour planes, we show that we can extend the system to also utilize green illumination, in addition to the blue and red. Applying the green colour as on-axis illumination yields redundant information for enhanced error correction, which is used to verify the gathered data, resulting in reliable coordinates as well as producing visually attractive images.
    Original languageEnglish
    JournalJournal of the European Optical Society - Rapid Publications
    Volume4
    Pages (from-to)09045
    ISSN1990-2573
    DOIs
    Publication statusPublished - 2009

    Keywords

    • three-dimensional microscopy
    • Optical trapping and manipulation

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