Multi-function microfluidic platform for sensor integration

Ana C. Fernandes*, Daria Semenova, Peter Panjan, Adama M. Sesay, Krist V. Gernaey, Ulrich Krühne

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The limited availability of metabolite-specific sensors for continuous sampling and monitoring is one of the main bottlenecks contributing to failures in bioprocess development. Furthermore, only a limited number of approaches exist to connect currently available measurement systems with high throughput reactor units. This is especially relevant in the biocatalyst screening and characterization stage of process development. In this work, a strategy for sensor integration in microfluidic platforms is demonstrated, to address the need for rapid, cost-effective and high-throughput screening in bioprocesses. This platform is compatible with different sensor formats by enabling their replacement and was built in order to be highly flexible and thus suitable for a wide range of applications. Moreover, this re-usable platform can easily be connected to analytical equipment, such as HPLC, laboratory scale reactors or other microfluidic chips through the use of standardized fittings. In addition, the developed platform includes a two-sensor system interspersed with a mixing channel, which allows the detection of samples that might be outside the first sensor's range of detection, through dilution of the sample solution up to 10 times. In order to highlight the features of the proposed platform, inline monitoring of glucose levels is presented and discussed. Glucose was chosen due to its importance in biotechnology as a relevant substrate. The platform demonstrated continuous measurement of substrate solutions for up to 12 h. Furthermore, the influence of the fluid velocity on substrate diffusion was observed, indicating the need for in-flow calibration to achieve a good quantitative output.
Original languageEnglish
JournalNew Biotechnology
Volume47
Pages (from-to)8-17
ISSN1871-6784
DOIs
Publication statusPublished - 2018

Keywords

  • In-flow glucose monitoring
  • Microfluidics
  • Sample dilution
  • Screening platform
  • Sensor integration

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