Motion analysis of optically trapped particles and cells using 2D Fourier analysis

Martin Verner Kristensen, Peter Ahrendt, Thue Bjerring Lindballe, Otto Højager Attermann Nielsen, Anton P. Kylling, Henrik Karstoft, Alberto Imparato, Leticia Hosta Rigau, Brigitte Stadler, Henrik Stapelfeldt, Søren Rud Keiding

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    Abstract

    Motion analysis of optically trapped objects is demonstrated using a simple 2D Fourier transform technique. The displacements of trapped objects are determined directly from the phase shift between the Fourier transform of subsequent images. Using end-and side-view imaging, the stiffness of the trap is determined in three dimensions. The Fourier transform method is simple to implement and applicable in cases where the trapped object changes shape or where the lighting conditions change. This is illustrated by tracking a fluorescent particle and a myoblast cell, with subsequent determination of diffusion coefficients and the trapping forces.
    Original languageEnglish
    JournalOptics Express
    Volume20
    Issue number3
    Pages (from-to)1953-1962
    ISSN1094-4087
    DOIs
    Publication statusPublished - 2012

    Bibliographical note

    This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/abstract.cfm?URI=oe-20-3-1953. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

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