Monolayers of a De Novo Designed 4-Alpha-Helix Bundle Carboprotein and Partial Structures on Au(111)-Surfaces

Jesper Brask, Hainer Wackerbarth, Knud Jørgen Jensen, Jingdong Zhang, Jens Ulrik Nielsen, Jens Enevold Thaulov Andersen, Jens Ulstrup

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Mapping of structure and function of proteins adsorbed on solid surfaces is important in many contexts. Electrochemical techniques based on single-crystal metal surfaces and in situ scanning probe microscopies (SPM) have recently opened new perspectives for mapping at the single-molecule level. De novo design of model proteins has evolved in parallel and holds promise for test and control of protein folding and for new tailored protein structural motifs. These two strategies are combined in the present report. We present a synthetic scheme for a new 4alpha-helix bundle carboprotein built on a galactopyranoside derivative with a thiol anchor aglycon suitable for surface immobilization on gold. The galactopyranoside with thiol anchor and the thiol anchor alone were prepared for comparison. Voltammetry of the three molecules on Au(111) showed reductive desorption peaks caused by monolayer adsorption via thiolate-Au bonding. In situ STM of the thiol anchor disclosed an ordered adlayer with clear domains and molecular features. This holds promise, broadly for single-molecule voltammetry and the SPM and scanning tunnelling microscopy (STM) of natural and synthetic proteins.
Original languageEnglish
JournalBioelectrochemistry
Volume56
Issue number1-2
Pages (from-to)27-32
ISSN1567-5394
Publication statusPublished - 2002

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