Monoclonal antibodies for the detection of Puccinia striiformis urediniospores

Peter Skottrup, Hanne Frøkiær, Stephen Hearty, Richard O'Kennedy, Jørn Hejgaard, Mogens Nicolaisen, Annemarie Fejer Justesen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The fungal pathogen Pst causes yellow rust disease in wheat plants leading to crop losses. The organism spreads by releasing wind-dispersed urediniospores from infected plants. In this study a library of novel monoclonal antibodies (mAbs) was developed against Pst urediniospores. Nine mAb-producing cell lines were cloned and their cross-reactivities characterised against a panel of airborne fungal spores representing genera commonly found in the same environment as Pst. Two specific mAbs were used to develop a competitive ELISA (Pst mAb4) and a subtractive inhibition ELISA (Pst mAb8). Standard curves for both assays had good intra- and interday reproducibility. The subtractive inhibition ELISA had greater sensitivity with a detection limit of 1.5 105 spores ml1. Cross-reactivity studies of Pst mAb8 in the subtractive inhibition ELISA, showed reaction with other Puccinia spores only, suggesting that common epitopes exist within this genus. The biosensor-compatible Pst mAb8 assay principle developed in this study has the potential to be implemented in future ‘label-free’ in-the-field systems for Pst detection.
    Original languageEnglish
    JournalMycological Research
    Volume111
    Pages (from-to)332-338
    ISSN0953-7562
    DOIs
    Publication statusPublished - 2007

    Cite this

    Skottrup, P., Frøkiær, H., Hearty, S., O'Kennedy, R., Hejgaard, J., Nicolaisen, M., & Justesen, A. F. (2007). Monoclonal antibodies for the detection of Puccinia striiformis urediniospores. Mycological Research, 111, 332-338. https://doi.org/10.1016/j.mycres.2006.11.010