Mono-colonization with Lactobacillus acidophilus NCFM affects the intestinal metabolome in mice

Henrik Munch Roager, Karolina Sulek, Kasper Skov, Henrik Lauritz Frandsen, Jørn Smedsgaard, Andrea Wilcks, Thomas Hjort Skov, Silas Granato Villas-Boas, Tine Rask Licht

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Mono-colonization of germ-free (GF) mice enables the study of specific bacterial species in vivo. Lactobacillus acidophilus is a probiotic strain, however many of the mechanisms behind its health-promoting effect remain unsolved. Here, we studied the effects of Lactobacillus acidophilus NCFMTM (NCFM) on the intestinal metabolome (jejunum, caecum, and colon) in mice by comparing NCFM mono-colonized (MC) mice with GF mice using liquid chromatography coupled to mass-spectrometry (LC-MS). The study adds to existing evidence that NCFM in vivo affects the bile acid signature of mice by deconjugation and dehydroxylation of bile acids. Furthermore, we confirmed that carbohydrate metabolism is affected by NCFM in the mouse intestine. Especially, the digestion of larger carbohydrates (penta- and tetrasaccharides) was increased in MC mice. Interestingly, we also found vitamin E (α-tocopherol acetate) in higher levels in the intestine of GF mice compared to MC mice, suggesting that NCFM either metabolizes the compound or indirectly affects the absorption by changing the metabolome in the intestine. The use of NCFM to increase the uptake of vitamin E supplements in humans and animals is a highly relevant topic for further research.
Original languageEnglish
Publication date2013
Number of pages1
Publication statusPublished - 2013
Event4th Danish Symposium on Metabolomics - University of Copenhagen, Copenhagen, Denmark
Duration: 15 Nov 201315 Nov 2013
Conference number: 4


Conference4th Danish Symposium on Metabolomics
LocationUniversity of Copenhagen


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