Abstract
Mono-colonization of germ-free (GF) mice enables the study of specific bacterial species in vivo. Lactobacillus acidophilus is a probiotic strain, however many of the mechanisms behind its health-promoting effect remain unsolved. Here, we studied the effects of Lactobacillus acidophilus NCFMTM (NCFM) on the intestinal metabolome (jejunum, caecum, and colon) in mice by comparing NCFM mono-colonized (MC) mice with GF mice using liquid chromatography coupled to mass-spectrometry (LC-MS). The study adds to existing evidence that NCFM in vivo affects the bile acid signature of mice by deconjugation and dehydroxylation of bile acids. Furthermore, we confirmed that carbohydrate metabolism is affected by NCFM in the mouse intestine. Especially, the digestion of larger carbohydrates (penta- and tetrasaccharides) was increased in MC mice. Interestingly, we also found vitamin E (α-tocopherol acetate) in higher levels in the intestine of GF mice compared to MC mice, suggesting that NCFM either metabolizes the compound or indirectly affects the absorption by changing the metabolome in the intestine. The use of NCFM to increase the uptake of vitamin E supplements in humans and animals is a highly relevant topic for further research.
Original language | English |
---|---|
Publication date | 2013 |
Number of pages | 1 |
Publication status | Published - 2013 |
Event | 4th Danish Symposium on Metabolomics - University of Copenhagen, Copenhagen, Denmark Duration: 15 Nov 2013 → 15 Nov 2013 Conference number: 4 |
Conference
Conference | 4th Danish Symposium on Metabolomics |
---|---|
Number | 4 |
Location | University of Copenhagen |
Country/Territory | Denmark |
City | Copenhagen |
Period | 15/11/2013 → 15/11/2013 |