Monitoring the hydration of DNA self-assembled monolayers using an extensional nanomechanical resonator

Alberto Cagliani, Priscila Kosaka, Javier Tamayo, Zachary James Davis

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We have fabricated an ultrasensitive nanomechanical resonator based on the extensional vibration mode to weigh the adsorbed water on self-assembled monolayers of DNA as a function of the relative humidity. The water adsorption isotherms provide the number of adsorbed water molecules per nucleotide for monolayers of single stranded (ss) DNA and after hybridization with the complementary DNA strand. Our results differ from previous data obtained with bulk samples, showing the genuine behavior of these self-assembled monolayers. The hybridization cannot be inferred from the water adsorption isotherms due to the low hybridization efficiency of these highly packed monolayers. Strikingly, we efficiently detect the hybridization by measuring the thermal desorption of water at constant relativity humidity. This finding adds a new nanomechanical tool for developing a label-free nucleic acid sensor based on the interaction between water and self-assembled monolayers of nucleic acids.
    Original languageEnglish
    JournalLab on a Chip
    Volume12
    Issue number11
    Pages (from-to)2069-2073
    ISSN1473-0197
    DOIs
    Publication statusPublished - 2012

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